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http://krishi.icar.gov.in/jspui/handle/123456789/70241| Title: | Revelation of candidate genes and molecular mechanism of reproductive seasonality in female rohu (Labeo rohita Ham.) by RNA sequencing |
| Other Titles: | Not Available |
| Authors: | Sarika Jaiswal Samiran Nandi Mir Asif Iquebal Rahul Singh Jasrotia Sunita Patra Gayatri Mishra Uday Kumar Udit Dinesh Kumar Sahu U. B. Angadi Prem Kumar Meher Padmanav Routray Jitendra Kumar Sundaray Dhananjay Kumar Verma Paramananda Das Pallipuram Jayasankar Anil Rai Dinesh Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Freshwater Aquaculture ICAR::Indian Agricultural Statistics Research Institute |
| Published/ Complete Date: | 2021-09-22 |
| Project Code: | Not Available |
| Keywords: | Labeo rohita Circannual, Cyprinid Biological clock genes Marker Reproductive seasonality |
| Publisher: | Not Available |
| Citation: | Jaiswal, S., Nandi, S., Iquebal, M.A. et al. Revelation of candidate genes and molecular mechanism of reproductive seasonality in female rohu (Labeo rohita Ham.) by RNA sequencing. BMC Genomics 22, 685 (2021). https://doi.org/10.1186/s12864-021-08001-6 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Background Carp fish, rohu (Labeo rohita Ham.) is important freshwater aquaculture species of South-East Asia having seasonal reproductive rhythm. There is no holistic study at transcriptome level revealing key candidate genes involved in such circannual rhythm regulated by biological clock genes (BCGs). Seasonality manifestation has two contrasting phases of reproduction, i.e., post-spawning resting and initiation of gonadal activity appropriate for revealing the associated candidate genes. It can be deciphered by RNA sequencing of tissues involved in BPGL (Brain-Pituitary-Gonad-Liver) axis controlling seasonality. How far such BCGs of this fish are evolutionarily conserved across different phyla is unknown. Such study can be of further use to enhance fish productivity as seasonality restricts seed production beyond monsoon season. Result A total of ~ 150 Gb of transcriptomic data of four tissues viz., BPGL were generated using Illumina TruSeq. De-novo assembled BPGL tissues revealed 75,554 differentially expressed transcripts, 115,534 SSRs, 65,584 SNPs, 514 pathways, 5379 transcription factors, 187 mature miRNA which regulates candidate genes represented by 1576 differentially expressed transcripts are available in the form of web-genomic resources. Findings were validated by qPCR. This is the first report in carp fish having 32 BCGs, found widely conserved in fish, amphibian, reptile, birds, prototheria, marsupials and placental mammals. This is due to universal mechanism of rhythmicity in response to environment and earth rotation having adaptive and reproductive significance. Conclusion This study elucidates evolutionary conserved mechanism of photo-periodism sensing, neuroendocrine secretion, metabolism and yolk synthesis in liver, gonadal maturation, muscular growth with sensory and auditory perception in this fish. Study reveals fish as a good model for research on biological clock besides its relevance in reproductive efficiency enhancement. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | BMC Genomics |
| Volume No.: | 22 |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1186/s12864-021-08001-6 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/70241 |
| Appears in Collections: | AEdu-IASRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Revelation of candidate genes and molecular mechanism of reproductive seasonality in female rohu (Labeo rohita Ham.) by RNA sequencing.pdf | 1.42 MB | Adobe PDF | View/Open |
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