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http://krishi.icar.gov.in/jspui/handle/123456789/72587
Title: | Development and Characterization of Novel Polymorphic Microsatellite Loci in Freshwater Mussel Lamellidens marginalis (Lamarck, 1819) Using Next Generation Sequencing |
Other Titles: | Not Available |
Authors: | Varshney, S., Sagwekar, M.,Pavan-Kumar, A., Das, R., Gireesh-Babu, P., Chaudhari, A., Krishna, G. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
Published/ Complete Date: | 2020-08-01 |
Project Code: | Not Available |
Keywords: | Simple sequence repeats; bivalves; pearl mussel; stock management |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The freshwater bivalve Lamellidens marginalis is native to Southeast Asia and one of the most widely used mussels for pearl production in the region. No studies have been carried out on stock characterisation of L. marginalis due to the lack of molecular markers. In the present study, the genome of L. marginalis was sequenced at low coverage using an Illumina pairedend approach. A total of 268,403 contigs with a size range of 258–13,308 bp was assembled from 2.5 million high quality reads. The contigs were mined and 26, 877 simple sequence repeats (SSRs) were identified using MIcroSAtellite identification tool software. Among SSRs, di-nucleotide repeats (15,633 total) were more frequent than the tri- (6306), tetra- (4768), penta- (146) or hexa-nucleotide (24) repeats. Eighteen loci representing di- (eight), tri- (seven) and tetra- (four) nucleotide repeats were characterised using 30 individuals of L. marginalis. All loci showed moderate to high Polymorphic Information Content values. There was no linkage disequilibria between any pair of loci. Fourteen microsatellites were successfully amplified in the closely related species Lamellidens corrianus. These polymorphic microsatellite markers will be useful for genetic selection programs, as well as for studying the population and conservation genetics of L. marginalis and L. corrianus. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Molluscan Research |
Journal Type: | International |
NAAS Rating: | 7.09 |
Impact Factor: | 1.088 |
Volume No.: | 40 (4) |
Page Number: | 308-312 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/72587 |
Appears in Collections: | FS-CIFE-Publication |
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