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http://krishi.icar.gov.in/jspui/handle/123456789/72711| Title: | Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton1822). |
| Other Titles: | Not Available |
| Authors: | Goswami, Chaudhari A, Nagpure NS, Gireesh-Babu P, Dubey A, Das DK |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
| Published/ Complete Date: | 2020-03-31 |
| Project Code: | Not Available |
| Keywords: | Danio rerio, zebrafish, Brahmaputra River, Assam, |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Danio rerio, zebrafish, has been widely used as a non-mammalian vertebrate model organism in various studies. The present research describes to develop and characterize a new cell line from a wild strain Indian zebrafish native to Brahmaputra River, Assam, India. The new cell line designated as DRCF was developed from the caudal fin of D. rerio. The cell line was successfully subcultured up to 31 passages. Growth studies revealed that cell growth of DRCF was optimal at 28 °C in L-15 medium supplemented with 20% FBS. Molecular characterization of the DRCF cell line using mitochondrial genes namely cytochrome oxidase subunit I gene (COI) and 16S rRNA authenticated the true origin of the cell line. The chromosome analysis of the DRCF cell line expressed its 50 diploid chromosome number of D. rerio. The immunocytochemical characterization of the cell line exhibited its fibroblastic morphology. The expression of the green fluorescent protein (GFP) following transfection revealed the suitability of the cell line for transfection studies. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Fish Physiology Biochemistry |
| Journal Type: | International |
| NAAS Rating: | 8.24 |
| Impact Factor: | 2.749 |
| Volume No.: | 46(4) |
| Page Number: | 1337-1347 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/72711 |
| Appears in Collections: | FS-CIFE-Publication |
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