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http://krishi.icar.gov.in/jspui/handle/123456789/72883
Title: | Nitrification potential of Achromobacter xylosoxidans isolated from freshwater finfish farms of Kerala, India |
Other Titles: | Not Available |
Authors: | Basha KA*, Joseph T, Lalitha KV, Prasad KP, D. Vineetha, G. Rathore, Gayatri Tripati |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
Published/ Complete Date: | 2018-07-11 |
Project Code: | Not Available |
Keywords: | Sediment, PCR, 16SrDNA, Achromobacter xylosoxidans, sodium pyruvate, Hetertrophic nitrification |
Publisher: | Excellent Publisher |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A heterotrophic strain ACM-1, isolated from Pangasius spp fish farms showed an ability to convert ammonium to nitrate under aerobic conditions. Based on the molecular phylogenetic analysis 16S rRNA gene sequence ACM-1 matched similarity to Achromobacter xylosoxidans (100%). ACM-1 showed amplification for Betaproteobacterial 16S AOB gene (465 bp) confirming the characteristic feature of ammonia oxidation. Sodium pyruvate (1%) was confirmed as the probable carbon source for the luxurious growth of Achromobacter xylosoxidans among the six carbon sources tested. At 30oC, 5% preculture and 120 rpm in shaker, ACM-1 could able to remove 100% ammonium within 36h. The ammonium removal rate was reported to be 1.3 NH4 + -N mg L -1 h -1 at 30oC. The rate of ammonium removal at different temperature (20oC, 25oC, 37oC) ranged between 0.9 to1.33 NH4 + -N mg L-1 h -1 . The nitrite produced during this experiment remained at constant rate and nitrate concentration increased after the initial hours of experiment. Therefore, the results demonstrate that Achromobacter xylosoxidans ACM-1 can remove ammonium in batch cultures and could be applied in aquaculture farms as a biological nitrifier. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | International Journal of Current Microbiology and Applied Sciences |
Journal Type: | International |
NAAS Rating: | 5.38 |
Volume No.: | 7(8) |
Page Number: | 2645-2654 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/72883 |
Appears in Collections: | FS-CIFE-Publication |
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