Evaluation of cellular induction, soluble components of proteins and expression of pro-inflammatory genes in Labeo rohita fingerlings.

Abstract

Methodology : Results : Interpretation : To investigate the acute inflammatory response in induced by carrageenan (red seaweeds) The fingerlings were injected with 50 examined at 3, 6, 12, 24, 48 and 96 hr post injection. The cellular markers that includes thrombocyte, macrophage, monocyte, granulocyte (neutrophils) and lymphocyte counts, serum myeloperoxidase activity, nitroblue tetrazolium (NBT) assay, total protein, albumin and globulin, C-reactive protein (CRP) and cortisol were examined. Additionally, the mRNA expression of proinflammatory cytokine genes IL-1 alyzed by real time PCR. Analysis of fish fingerlings showed significantly (< 0.05) higher thrombocyte and macrophage count in the inflammatory exudates of carrageenan injected group. The neutrophil and monocyte number were correlated with the inflammatory response and higher counts were observed during 12 hrs and beyond, whereas lymphocyte counts were lower in the treated group as compared to control. The NBT assay and myeloperoxidase activity that indicates the neutrophils activation were significantly (< 0.05) upregulated in the treatment group. The C-reactive protein (acute phase protein), globulin, proinflammatory genes, i.e., complement C3 were recorded significantly (< 0.05) higher in the fishes injected with carrageenan as compared to control. The carrageenan induces acute inflammatory response in and the results provide a basic experimental model to further study the activity of anti-inflammatory drugs and mediators of inflammation. Labeo rohita Labeo rohita p p p Labeo rohita μl of carrageenan and the inflammatory response was β and TNF-α and complement (C3) were an IL-1β, TNF-α and