Assessment of DNA damage during gene delivery in freshwater prawn by chitosan reduced gold nanoparticles.

Abstract

The increasing application of nanoparticles both in industries and in agricultural fields has led to its accumulation in the aquatic ecosystem through water run-off. Insights into the validity of safer nanoparticles such as gold and chitosan are fairly established. However, its effect on aquatic invertebrates has been less studied. The present study was aimed to study effects of chitosan reduced gold nanoparticles (CRGNPs) during green fluorescent protein (GFP) encoding plasmid delivery in giant freshwater prawn, macrobrachium rosenbergii. The mean particle size and zeta potential CRGNPs was 33.7 nm and 24.79 mV respectively. Prawn juveniles were exposed to nanoparticles concentrations (10 µg/L, 20 µg/L) of CRGNPs by immersion treatment for a period of 36 hours. GFP was ubiquitously expressed in muscle tissues of prawns. The comet assay indicated dose dependent genotoxicity of CRGNPs in gill, pleopod and muscle tissues which was in conformity with its bioaccumulation pattern in vivo. The highest bioaccumulation of CRGNPs was found in Gills, followed by pleopods and least in muscles. Hence, the toxicological potential of CRGNPs to the environment cannot be denied and demands more research on the particular aspect. The doses standardized in the present study would be helpful in safer nano-gene delivery in aquatic invertebrates and development of transgenics employing less cost.