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http://krishi.icar.gov.in/jspui/handle/123456789/73148| Title: | Monoclonal antibodies against recombinant GAPDH of Edwardsiella tarda reveal the conserved nature of the protein. |
| Other Titles: | Not Available |
| Authors: | Banu H, Anand D, Bedekar MK, Rajendran KV, Makesh M |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
| Published/ Complete Date: | 2017-03-11 |
| Project Code: | Not Available |
| Keywords: | Edwardsiella Tarda, Outer Membrane Protein, GAPDH, Monoclonal Antibodies |
| Publisher: | Taylor & Francis |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The outer membrane protein, encoded by glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, of Edwardsiella tarda is a highly conserved immunogenic protein. The GAPDH was cloned and expressed in Escherichia coli. The purified protein was used to produce mouse monoclonal antibodies (MAbs). Four stable hybridomas producing MAbs (3G12, 4E9, 5A11 and 9G1) against rGAPDH were obtained. The heavy chains of antibodies produced by the hybridomas were of the isotypes IgG1 and IgM. Cross reactivity of MAbs (3G12 and 9G1) was observed with GAPDH of Aeromonas hydrophila and Micrococcus luteus. MAbs 3G12 and 4E9 reacted with Vibrio cholerae, Salmonella enterica and Penaeus monodon tissues but not with vertebrate GAPDH. None of the MAbs reacted with Staphylococcus aureus. The results indicate that the level of conservation of GAPDH is high among evolutionarily close species. The MAbs developed will be a useful tool to study the evolutionarily conserved and functionally diverse GAPDH. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Food and Agricultural Immunology |
| Journal Type: | International |
| NAAS Rating: | 7.39 |
| Impact Factor: | 3.101 |
| Volume No.: | 28(4) |
| Page Number: | 685-698 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/73148 |
| Appears in Collections: | FS-CIFE-Publication |
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