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http://krishi.icar.gov.in/jspui/handle/123456789/7452
Title: | Simple and rapid system for two‐dimensional gel electrophoresis technique: A laboratory exercise for high school students |
Other Titles: | Not Available |
Authors: | Praveen Maurye, Arpita Basu, Jayanta Kumar Biswas, Tapas Kumar Bandyopadhyay, Malay Naskar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Central Inland Fisheries Research Institute (I.C.A.R.), Barrackpore, Kolkata, West Bengal 700 120. India. |
Published/ Complete Date: | 2015-05-01 |
Project Code: | Not Available |
Keywords: | 2D-PAGE, proteins, proteomics, training programme |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Polyacrylamide gel electrophoresis (PAGE) is the most classical technique favored worldwide for resolution of macromolecules in many biochemistry laboratories due to its incessant advanced developments and wide modifications. These ever‐growing advancements in the basic laboratory equipments lead to emergence of many expensive, complex, and tricky laboratory equipments. Practical courses of biochemistry at high school or undergraduate levels are often affected by these complications. Two dimensional gel electrophoresis technique (2D‐PAGE) used for resolving thousands of proteins in a gel is a combination of isoelectric focusing (first dimension gel electrophoresis technique) and sodium‐dodecylsulphate PAGE (second dimension gel electrophoresis technique or SDS‐PAGE). Two different laboratory equipments are needed to carry out effective 2D‐PAGE technique, which also invites extra burden to the school laboratory. Here, we describe a low cost, time saving and simple gel cassette for protein 2D‐PAGE technique that uses easily fabricated components and routine off‐the‐shelf materials. The performance of the apparatus was verified in a practical exercise by a group of high school students with positive outcomes. © 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):237–244, 2018. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biochemistry and Molecular Biology Education |
NAAS Rating: | 6.92 |
Volume No.: | 46 |
Page Number: | 237-244 |
Name of the Division/Regional Station: | Fishery Resource and Environment Management Division |
Source, DOI or any other URL: | https://doi.org/10.1002/bmb.21116 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7452 |
Appears in Collections: | FS-CIFRI-Publication |
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