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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/74644
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shashi Kant Kankar, Sachin Kumar Goyal, Parvati Sharma, Deepak Kumar Gaur, Souti Prasad Sarkhel and Rajender Kumar | en_US |
dc.date.accessioned | 2022-09-29T09:53:20Z | - |
dc.date.available | 2022-09-29T09:53:20Z | - |
dc.date.issued | 2013-07-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/74644 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Trypanosoma evansi (T. evansi) is a causative agent of disease called surra, affecting wide range of domestic and wild animals. In this study, a PCR assay was developed using primers targeting ITS-1 region flaking between 5.8 S and 18 S subunits of rRNA. The test was employed using serially diluted DNA extracted from purified trypanosomes ranging from 200 ng/μl to 0.00002 pg/μl and T. evansi infected mice blood ranging from 350 ng/μl to 0.00035 ng/μl. The diagnostic sensitivity of PCR assay was found to be 0.2 pg/μl and 0.035 ng/μl with purified parasite DNA and infected mice blood DNA, respectively. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Indian Journal.com | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Trypanosma evansi, Surra, ITS-1, PCR, rDNA, Equine | en_US |
dc.title | PCR-based Identification of Trypanosoma evansi targeting Internaltranscribed Spacer 1 of rDNA in experimentally infected Mice | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Immunology and Immunopathology | en_US |
dc.publication.volumeno | 15(2) | en_US |
dc.publication.pagenumber | 210-214 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.5958/0973-9149.2014.01070.3 | en_US |
dc.publication.authorAffiliation | ICAR::National Research Centre on Equines | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | AS-NRCE-Publication |
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