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http://krishi.icar.gov.in/jspui/handle/123456789/74872| Title: | An expedient ion chromatography based method for high‐throughput analysis of phytic acid in groundnut kernels |
| Other Titles: | Not Available |
| Authors: | Aman Verma Sushmita Singh L.K. Thawait Mahesh K. Mahatma A.L. Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Arid Zone Research Institute ICAR::Directorate of Groundnut Research ICAR::National Research Centre on Seed Spices |
| Published/ Complete Date: | 2022-11-01 |
| Project Code: | Not Available |
| Keywords: | Absorption Phytic acid |
| Publisher: | Not Available |
| Citation: | Aman Verma, Sushmita Singh, Lokesh K. Thawait, Mahesh K. Mahatma and AL Singh (2022) An expedient ion chromatography based method for high‐throughput analysis of phytic acid in groundnut kernels. Journal of Food Science and Technology. 59: 4479-4486. https://doi.org/10.1007/s13197-022-05527-9 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A study was made to expedite ion chromatography method using IonPac analytical column and self regenerating anion suppressor for phytic acid determination in groundnut seeds and compared with widely adopted spectrophotometric method based on enzymatic hydrolysis. The Ion Chromatography method equipped with AG11 guard and AS11 analytical columns in isocratic mode using 0.65 mM NaOH mobile phase at 1 mL min-1 flow rate showed a sharp peak for phytic acid with a retention time of 2.42±0.2 min. The peak area were plotted v/s concentration showed linearity with R2 value of 0.997, detection limit of 0.028 mg L-1 and recovery of 98% as against R2 value of 0.988 and detection limit of 0.065 mg L-1 in spectrophotometric method. The study clearly demonstrates that Ion Chromatography method was more accurate with better detection limit than spectrophotometry. Also, this method provides powerful handling with lesser reagent requirement due to combined eluent generation and self-regenerating suppression. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Food Science and Technology |
| NAAS Rating: | 8.79 |
| Volume No.: | 59 |
| Page Number: | 4479-4486 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s13197-022-05527-9 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/74872 |
| Appears in Collections: | NRM-CAZRI-Publication |
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