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http://krishi.icar.gov.in/jspui/handle/123456789/80377| Title: | Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India. |
| Other Titles: | Not Available |
| Authors: | Kaur,P Filia,G Singh,S.V Patil,P.K Ravi Kumar,G.V.P.P.S. Sandhu,K.S. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Brackishwater Aquaculture |
| Published/ Complete Date: | 2010-01-01 |
| Project Code: | Not Available |
| Keywords: | Johne’s disease Mycobacterium avium subspecies paratuberculosis Ziehl–Neelsen staining PCR IS900 IS1311 REA Molecular epidemiology |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Johne’s disease is chronic granulomatous infectious enteritis of animals caused by Mycobac terium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from dif ferent districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected to Ziehl–Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl–Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’ type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’ type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of ‘Cattle’ type Map from buffalo and goat species in India |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/80377 |
| Appears in Collections: | FS-CIBA-Publication |
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