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http://krishi.icar.gov.in/jspui/handle/123456789/81562
Title: | Cloning and comparative modeling identifies a highly stress tolerant Cu/Zn cytosolic super oxide dismutase 2 from a drought tolerant maize inbred line |
Other Titles: | Not Available |
Authors: | Anuradha Gautam Fatima Nazish Khan Surabhi Priya Krishan Kumar Shivani Sharda Tanushri Kaul Ishwar Singh Sapna Langyan Pranjal Yadava |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Indian Institute of Maize Research, Pusa Campus, New Delhi, India Division of Plant Physiology, Indian Agricultural Research Institute, Pusa, New Delhi, India Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, India Amity Institute of Biotechnology, Amity University, Noida, India International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India Division of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, India |
Published/ Complete Date: | 2023-03-13 |
Project Code: | Not Available |
Keywords: | Zea mays L. Superoxide dismutase SOD2 Drought stress Bioinformatic analysis |
Publisher: | Peer J |
Citation: | Not AvGautam A, Khan FN, Priya S, Kumar K, Sharda S, Kaul T, Singh I, Langyan S, Yadava P. 2023. Cloning and comparative modeling identifies a highly stress tolerant Cu/Zn cytosolic super oxide dismutase 2 from a drought tolerant maize inbred line. PeerJ 11:e14845 DOI 10.7717/peerj.14845ailable |
Series/Report no.: | Not Available; |
Abstract/Description: | Plants have a complex system of stress response that deals with different types of stresses. Maize (Zea mays L.), one of the most important crops grown throughout the world, across a range of agro-ecological environments, employs complex mechanisms of gene regulation in response to drought stress. HKI 335 is a tropical maize inbred line showing remarkable adaptation to drought stress. Abiotic stresses, like drought, trigger the production of reactive oxygen species (ROS) due to the incomplete reduction or excitation of molecular oxygen, eventually leading to cell damage. Superoxide dismutase (SOD, EC 1.15.1.1) is a metalloenzyme that acts as the first line of defense against ROS. We cloned the Sod2 gene from HKI 335 inbred line and analyzed its protein through detailed in silico characterization. Our comparative modeling revealed that at the level of tertiary structure, the HKI 335 SOD2 protein is highly similar to Potentilla atrosanguinea SOD2, which had been previously identified as highly thermostable SOD that can tolerate autoclaving as well as sub-zero temperatures. We performed phylogenetic analysis, estimated physicochemical properties, post-translational modifications, protein-protein interactions, and domain composition of this SOD2. The phylogenetic analysis showed that orthologous sequences of SOD from different species were clustered into two clusters. Secondary structure prediction indicates that SOD2 is a soluble protein and no transmembrane domains have been found. Most of the beta sheets have RSA value greater than 2. The Ramachandran plot from PDBsum revealed that most of the residues fall in the highly favored region. It was estimated that the value of the instability index was less than 40, the value of the aliphatic index was extremely high and the GRAVY value lies between −2 and +2. We could identify only one phosphorylation site, located at position 20 with a score of 0.692. Overall, the unique stress-tolerant properties of the HKI 335 SOD2, may be one of the reasons contributing to the high drought tolerance trait exhibited by HKI 335 maize inbred line. Further research may reveal more insights into the drought adaptation mechanism in maize and the eventual deployment of the trait in maize hybrids. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Peer J |
Journal Type: | Not Available |
NAAS Rating: | 9 |
Impact Factor: | 3 |
Volume No.: | 11:e14845 |
Page Number: | 1-18 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.7717/peerj.14845 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/81562 |
Appears in Collections: | CS-IIMR-Publication |
Files in This Item:
File | Description | Size | Format | |
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peerj-14845.pdf | 19.02 MB | Adobe PDF | View/Open |
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