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Title: | Multiplex molecular marker-assisted analysis of significant pathogens of cotton (Gossypium sp.), Biocatalysis and Agricultural Biotechnology |
Other Titles: | Not Available |
Authors: | Chavhan, R.L., Sable, S., Narwade, A.V., Hinge, V.R., Kalbande, B.B., Mukherjee, A.K., Chakrabarty, P.K., Kadam, U.S |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR National Rice Research Instititute |
Published/ Complete Date: | 2022-11-19 |
Project Code: | Not Available |
Keywords: | rDNA CAPS, Cotton Pathogen diagnosis, Multiplex-PCR, Disease control, Molecular biodiagnostics, Molecular markers |
Publisher: | Elsevier |
Citation: | Chavhan, R.L., Sable, S., Narwade, A.V., Hinge, V.R., Kalbande, B.B., Mukherjee, A.K., Chakrabarty, P.K., Kadam, U.S. (2023). Multiplex molecular marker-assisted analysis of significant pathogens of cotton (Gossypium sp.), Biocatalysis and Agricultural Biotechnology, 47:102557;doi:https://doi.org/10.1016/j.bcab.2022.102557 |
Series/Report no.: | Not Available; |
Abstract/Description: | Plant pathogens diminish crop quality and yield, leading to economic losses. Molecular markers have the potential for early and accurate detection of pathogens. DNA markers based on ITS regions, pthN gene, and CP gene were designed to detect strains of fungal, bacterial, and viral pathogens, respectively. The pRS and pRB primers were found specific to strains of R. solani and R. bataticola. Four strains of R. areola isolates from each cultivated cotton species were detected using the primer pRARE. Further, CAPS markers, Bst6I, and DraRI specific to the strains of Alternaria macrospora were developed, which could differentiate from other fungal pathogens. Furthermore, we developed a multiplexed assay to detect six pathogens simultaneously. These diagnostic markers could help assess the prevalence of cotton infecting pathogens in the field (from plant tissue or soil). Consequently, the diagnostic assay could be used for undertaking judicious disease control measures in commercial agriculture. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Multiplex molecular marker-assisted analysis of significant pathogens of cotton (Gossypium sp.) |
Journal Type: | Not included in NAAS Journal List |
NAAS Rating: | Not Available |
Impact Factor: | 4.0 |
Volume No.: | 47(102557) |
Page Number: | Not Available |
Name of the Division/Regional Station: | Division of Crop Protection |
Source, DOI or any other URL: | https://doi.org/10.1016/j.bcab.2022.102557 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/81765 |
Appears in Collections: | CS-NRRI-Publication |
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