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http://krishi.icar.gov.in/jspui/handle/123456789/82552| Title: | Strategies to improve genome editing efficiency in crop plants |
| Other Titles: | Not Available |
| Authors: | B. Aravind Kutubuddin Molla Satendra K. Mangrauthia Gireesha Mohannath |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Birla Institute of Technology and Science ICAR-National Rice Research Institute ICAR-Indian Institute of Rice Research |
| Published/ Complete Date: | 2023-11-08 |
| Project Code: | Not Available |
| Keywords: | Genome editing CRISPR-Cas Transgene-free Viral vectors Histone deacetylase inhibitors TRBO vector |
| Publisher: | Springer-Verlag |
| Citation: | Aravind, B., Molla, K., Mangrauthia, S. K., & Mohannath, G. (2023). Strategies to improve genome editing efficiency in crop plants. Journal of Plant Biochemistry and Biotechnology, 32(4), 661-672. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Genome editing technology comprises site-directed mutagenesis of genomes, involving alterations of few bases to precise replacement of a fragment or an entire gene sequence. Among multiple types of genome editing technologies developed, CRISPR-Cas9 and its latest variants have been revolutionizing the field of genetic engineering and plant biotechnology. Despite several advantages the CRISPR-Cas9 technology offers, it often suffers from low efficiency in creating desirable mutants in several crop plant species. In this review, we discuss various emerging strategies to improve genome editing efficiency in crop plants. The strategies include increased expression of genome editing components using high efficiency viral vectors, employment of inhibitors of chromatin modifiers, and using plant DNA viruses as donor DNA carriers. Additionally, we also discuss strategies to obtain transgene-free genome edited crops. |
| Description: | Review Paper |
| ISSN: | Not Available |
| Type(s) of content: | Review Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
| Journal Type: | Not Available |
| NAAS Rating: | 7.53 |
| Impact Factor: | 1.9 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Crop Improvement Division |
| Source, DOI or any other URL: | https://link.springer.com/article/10.1007/s13562-023-00860-2#:~:text=The%20strategies%20include%20increased%20expression,viruses%20as%20donor%20DNA%20carriers. |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/82552 |
| Appears in Collections: | CS-NRRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Strategies to improve genome editing efficiency in crop plants.pdf | 1.14 MB | Adobe PDF | View/Open |
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