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http://krishi.icar.gov.in/jspui/handle/123456789/8624
Title: | Molecular identification of dominant cultivars of soybean using simple sequence repeat marker. |
Other Titles: | Not Available |
Authors: | Vineet Kumar, Anita Rani Priyamvada Jha, Reena Rawal S.M. Husain |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR: Indian Institute of Soybean Research |
Published/ Complete Date: | 2017-07 |
Project Code: | Not Available |
Keywords: | Simple sequence repeat Varieties identification Soybean |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Molecular identity of dominant cultivars of soybean needs to complement the morphological descriptors, which are insufficient and influenced by environment, for protecting the interest of breeders and maintaining the genetic purity. In the present investigation, genomic DNA of six commercial Indian soybean varieties viz. JS95-60, JS93-05, NRC7, JS97-52, NRC37, JS335 was amplified using ten simple sequence repeat (SSR) markers, which were selected based upon the relatively higher polymorphic information content (PIC) value observed after polymorphism survey of 46 soybean genotypes through 58 SSR markers. PCR analysis showed nine polymorphic SSR primers which could differentiate unambiguously all the six varieties. Maximum similarity was observed between NRC7, JS93-05 and JS95-60. Two unique alleles amplified in each of the two varieties namely JS335 and NRC37 and four unique alleles in JS97-52 unambiguously differentiated them from the remaining varieties. Morphologically similar varieties JS93-05 and JS95-60 could be differentiated distinctly by primer pair Satt181, while NRC7 could be distinguished from both JS93-05 and JS95-60 through Sct_199. Results also showed that these nine polymorphic markers could distinguish the dominant cultivars from six non-trading varieties, which further confirmed the utility of nine SSR primers for protecting the breeders’ rights and maintaining the purity of dominant cultivars during seed production programme |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Proceeding of National Academy of Sciences , India , Section B Biological Sciences |
Volume No.: | 87(3) |
Page Number: | 647-653 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI 10.1007/s40011-015-0641-7 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8624 |
Appears in Collections: | CS-DSBR-Publication |
Files in This Item:
File | Description | Size | Format | |
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PNAS Dominant soy varieties through SSR markers.pdf | 691.59 kB | Adobe PDF | View/Open |
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