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http://krishi.icar.gov.in/jspui/handle/123456789/9543| Title: | Sorghum grain induced disruption of gut amylases in lesser grain weevil Sitophilus oryzae (L). |
| Other Titles: | Not Available |
| Authors: | Padmaja, P.G., Shwetha, B.L., Kalaisekar, A., Shyam Prasad, G., Srinivas babu and Tonapi, V.A |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Millets Research |
| Published/ Complete Date: | 2018-04-12 |
| Project Code: | Not Available |
| Keywords: | Sorghum grain, gut amylases, Sitophilus oryzae (L) |
| Publisher: | Sorghum in the 21st Century, Cape Town, South Africa |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Sorghum grain induced disruption of gut amylases in lesser grain weevil Sitophilus oryzae (L) PG Padmaja, BL Shwetha, A Kalaisekar, K Srinivasa babu, G Shyamprasad, VA Tonapi ICAR - Indian Institute of Millets Research, Hyderabad, India. Insects that breed in stored grains are largely banking on the gut amylase enzymes for the digestion of starchy food. Lesser grain weevil, Sitophilus oryzae is an important storage pest on sorghum grains. Our present study evaluates α-amylase inhibitory activity in released sorghum varieties and hybrids. Adult S. oryzae were obtained from a laboratory stock culture maintained at 27 ± 10°C, 70 % RH, and a photoperiod of 12:12 (L:D) h at ICAR- Indian Institute of Millets Research, Rajendranagar, Hyderabad, India. Seven sorghum genotypes viz., CSV 15, CSV 26, CSV 24SS, CSH 16, Phule Suchitra, Phule Revathi and Phule Vasudha were tested for α - amylase inhibition. We observed significant variation in the number of adults of S. oryzae emerged from different cultivars of sorghum grains in laboratory cultures. Among the tested genotypes, the lowest number of adult emergence was observed in CSV 26 (173 adults) and CSV24SS (185 adults) while highest numbers observed in CSV 15 (421 adults). Our analysis revealed that significantly higher amylase inhibition activity of 72 and 74% in the sorghum grains of CSV 26 and CSV 24SS respectively. We also found two α-amylase isozymes in S. oryzae which are predominant enzymes for the starch digestion. Increased α-amylase activity in the insect gut had a corresponding rise in the percent amylase inhibition. This increase in amylase activity in the insect gut could be in response to the higher inhibition activity in the sorghum extracts. In the present study, α- amylase inhibitors prepared from sorghum showed a potential as pest resistance factor against S. oryzae. The reduction of α- amylase activity by extracts could be due to the plant defense compounds that act on insect gut enzymes, α-amylases and proteinases. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Presentation |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9543 |
| Appears in Collections: | CS-IIMilletsR-Publication |
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