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Cytotechnology

, Volume 68, Issue 6, pp 2271–2285 | Cite as

Developmental competence and expression profile of genes in buffalo (Bubalus bubalis) oocytes and embryos collected under different environmental stress

  • E. M. SadeeshEmail author
  • P. Sikka
  • A. K. Balhara
  • S. Balhara
Original Article
First Online:

Abstract

The study examined the effects of different environmental stress on developmental competence and the relative abundance (RA) of various gene transcripts in oocytes and embryos of buffalo. Oocytes collected during cold period (CP) and hot period (HP) were matured, fertilized and cultured in vitro to blastocyst hatching stage. The mRNA expression patterns of genes implicated in developmental competence (OCT-4, IGF-2R and GDF-9), heat shock (HSP-70.1), oxidative stress (MnSOD), metabolism (GLUT-1), pro-apoptosis (BAX) and anti-apoptosis (BCL-2) were evaluated in immature and matured oocytes as well as in pre-implantation stage embryos. Oocytes reaching MII stage, cleavage rates, blastocyst yield and hatching rates increased (P < 0.05) during the CP. In MII oocytes and 2-cell embryos, the RA of OCT-4, IGF-2R, GDF-9, MnSOD and GLUT-1 decreased (P < 0.05) during the HP. In 4-cell embryos, the RA of OCT-4, IGF-2R and BCL-2 decreased (P < 0.05) in the HP, whereas GDF-9 increased (P < 0.05). In 8-to 16-cell embryos, the RA of OCT-4 and BCL-2 decreased (P < 0. 05) in the HP, whereas HSP-70.1 and BAX expression increased (P < 0.05). In morula and blastocyst, the RA of OCT-4, IGF-2R and MnSOD decreased (P < 0.05) during the HP, whereas HSP-70.1 was increased (P < 0.05). In conclusion, deleterious seasonal effects induced at the GV-stage carry-over to subsequent embryonic developmental stages and compromise oocyte developmental competence and quality of developed blastocysts.

Keywords

Seasonality Oocyte quality Gene expression In vitro embryo production Embryo development 
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Notes

Acknowledgments

Funding support from Indian Council of Agricultural Research (ICAR), New Delhi is gratefully acknowledged. The authors would like to thank Dr. Inderjeet Singh, Director, ICAR-Central Institute for Research on Buffaloes, for providing the necessary facilities for conducting this research. We are also thankful to Dr. Dana Thomsen (The University of Adelaide, Adelaide, Australia), for reviewing this manuscript.

Authors’ contributions

EMS conceived of the study, carried out the experiments and drafted the manuscript. PS, AKB and SB assisted with the sample analysis. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10616_2016_22_MOESM1_ESM.doc (15.5 mb)
Supplementary material 1 (DOC 15827 kb)

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© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • E. M. Sadeesh
    • 1
    Email author
  • P. Sikka
    • 2
  • A. K. Balhara
    • 1
  • S. Balhara
    • 2
  1. 1.Division of Animal Physiology and ReproductionICAR-Central Institute for Research on BuffaloesHisarIndia
  2. 2.Division of Animal Genetics and BreedingICAR-Central Institute for Research on BuffaloesHisarIndia

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