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http://krishi.icar.gov.in/jspui/handle/123456789/1118| Title: | Evaluation of different protein extraction methods for banana (Musa spp.) root proteome analysis by two-dimensional electrophoresis |
| Other Titles: | Not Available |
| Authors: | Mayil vaganan M., Sarumathi, S., Nandakumar, A., Ravi, I. and Mustaffa, M.M. |
| Published/ Complete Date: | 2015-02-01 |
| Project Code: | Not Available |
| Keywords: | Evaluation of different protein, extraction methods, root proteome analysis, two-dimensional electrophoresis |
| Publisher: | PubMed |
| Citation: | 0 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Four protocols viz., the trichloroacetic acid-acetone (TCA), phenol-ammonium acetate (PAA), phenol/SDS-ammonium acetate (PSA) and trisbase-acetone (TBA) were evaluated with modifications for protein extraction from banana (Grand Naine) roots, considered as recalcitrant tissues for proteomic analysis. The two-dimensional electrophoresis (2-DE) separated proteins were compared based on protein yield, number of resolved proteins, sum of spot quantity, average spot intensity and proteins resolved in 4-7 pI range. The PAA protocol yielded more proteins (0.89 mg/g of tissues) and protein spots (584) in 2-DE gel than TCA and other protocols. Also, the PAA protocol was superior in terms of sum of total spot quantity and average spot intensity than TCA and other protocols, suggesting phenol as extractant and ammonium acetate as precipitant of proteins were the most suitable for banana rooteomics analysis by 2-DE. In addition, 1:3 ratios of root tissue to extraction buffer and overnight protein precipitation were most efficient to obtain maximum protein yield. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Biochemistry and Biophysics |
| NAAS Rating: | 6.54 |
| Volume No.: | 52(2) |
| Page Number: | 101-6 |
| Name of the Division/Regional Station: | Crop Production |
| Source, DOI or any other URL: | https://www.ncbi.nlm.nih.gov/pubmed/26040117 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/1118 |
| Appears in Collections: | HS-NRCB-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Evaluation of different protein extraction methods in banana_Root proteome analysis.pdf | 190.94 kB | Adobe PDF | View/Open |
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