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http://krishi.icar.gov.in/jspui/handle/123456789/11544
Title: | Experimental infection of Betanodavirus in freshwater fish Gambusia affinis (Baird and Girard, 1853)—a potential infection model for viral encephalopathy and retinopathy |
Other Titles: | Not Available |
Authors: | J. Praveenraj P. Ezhil Praveena T. Bhuvaneswari A. Navaneeth Krishnan KP. Jithendran |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Brackishwater Aquaculture |
Published/ Complete Date: | 2018-01-02 |
Project Code: | Not Available |
Keywords: | Viral nervous necrosis, Betanodavirus,Gambusia affinis,Experimental transmission,RT-PCR.Histopathology, Electron microscopy |
Publisher: | CIBA |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Betanodaviruses are the causative agents of the disease known as viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) in a variety of marine and freshwater fish species. The aim of this study was to demonstrate experimental infection of an isolate of betanodavirus (RGNNV genotype) in freshwater fish,Gambusia affinis,for elucidation of transmission mechanism and potential use as a laboratory model. Morbidity and mortality rate was significantly higher by injection route of infection as compared to immersion by bath and resembled the natural infection of juvenile marine fish.The fish in disease affected group showed severe neurological disorders accompanied by extensive vacuolar degeneration and mild to moderate neuronal necrosis of the brain in comparison to control. Amplification of ~427 bp product in the variable region of the coat protein gene of betanodavirus was achieved by RT-PCR with 100% sequence homology to RGNNV genotype. |
Description: | Not Available |
ISSN: | 0967-6120 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Aquaculture international |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/Publication/handle/123456789/11544 |
Appears in Collections: | FS-CIBA-Publication |
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