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http://krishi.icar.gov.in/jspui/handle/123456789/13549
Title: | Development, distribution and expression of a DNA vaccine against nodavirus in Asian Seabass, Lates calcarifier (Bloch, 1790) |
Other Titles: | Not Available |
Authors: | R. Subburaj |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Central Institute of Brackishwater Aquaculture |
Published/ Complete Date: | 2014-09-01 |
Project Code: | Not Available |
Keywords: | betanodavirus, DNA vaccine, distribution, expression, neutralization |
Publisher: | CIBA |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Fish nodavirus (betanodavirus), a viral pathogen responsible for viral nervous necrosis (VNN) was isolated from infected Asian sea bass (Lates calcarifer). The distribution, clearance and expression of nodavirus vaccine, on the basis of DNA vaccine (pFNCPE42 DNA-pcDNA3.1) construction, were analysed in tissues of the Asian seabass by PCR, RT-PCR, ELISA and Immunohistochemistry. Fish immunized with a single intramuscular injection of 20 lg of the pFNCPE42-DNA vaccine showed a significant increase in the serum antibody level in the 3rd week after vaccination, compared to control eukaryotic expression vector pcDNA3.1 vaccinated fish. Results from PCR studies indicated that the vaccine-containing plasmids were distributed in heart, intestine, gill, muscle and liver 10 days after vaccination. Clearance of pFNCPE42-DNA vaccine was studied at 10, 25, 50, 75 and 100 days of post vaccination (d p.v). At 100 days p.v. pFNCPE42- DNA was cleared from muscle of vaccinated sea bass. In vitro and in vivo expression of fish nodavirus capsid protein gene (FNCP) was determined by fluorescent microscopy. Asian seabass was immunized with pFNCPE42-DNA vaccine at a dose of 20 lg per fish and were challenged with betanodavirus by intramuscular injection. The vaccinated seabass was protected from nodaviral infection and 77.33% of relative percent survival (RPS) was recorded. |
Description: | Not Available |
ISSN: | 1355-557X |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Aquaculture Research |
NAAS Rating: | 7.75 |
Volume No.: | Not Available |
Page Number: | 1-12 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13549 |
Appears in Collections: | FS-CIBA-Publication |
Files in This Item:
File | Description | Size | Format | |
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Aquaculture Research.pdf | 627.89 kB | Adobe PDF | View/Open |
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