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http://krishi.icar.gov.in/jspui/handle/123456789/15683| Title: | Development of chitosan conjugated DNA vaccine against nodavirus in Macrobrachiumrosenbergii (De Man, 1879) |
| Other Titles: | Not Available |
| Authors: | Ramya, V. L. Sharma, Gireesh-Babu, P. Patchala, S. R. Rather, A. Nandanpawar, P. C. Eswaran, S. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Education |
| Published/ Complete Date: | 2013-01 |
| Project Code: | Not Available |
| Keywords: | DNA Macrobrachium rosenbergii chitosan nodavirus |
| Publisher: | Not Available |
| Citation: | Ramya, V. L., Sharma, Gireesh-Babu, P., Patchala, S. R., Rather, A., Nandanpawar, P. C. andEswaran, S., 2013. Development of chitosan conjugated DNA vaccine against nodavirus in Macrobrachiumrosenbergii (De Man, 1879). Journal of Fish Diseases 37(9): 1-10 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The protective efficacy of a DNA construct containing extra small virus antisense (XSVAS) gene of nodavirus encapsulated with chitosan nanoparticles (NPs) was investigated in giant freshwater prawn Macrobrachium rosenbergii (De Man, 1879). The delivery was carried out using oral and immersion methods. A plasmid concentration of 100 ng μL(-1) when conjugated with chitosan NPs was found to be more effective in increasing the survivability of the infected prawn. The particle mean size, zeta potential and loading efficiency percentage were 297 nm, 27 mV and 85%, respectively. The ability of the chitosan to form a complex with the plasmid was studied by agarose gel electrophoresis. The NPs were characterized by atomic force microscopy (AFM). Persistence study showed the presence of the DNA construct up to 30th day post-treatment. The oral treatment was found to be better than the immersion treatment for delivery of the chitosan-conjugated DNA construct. This is probably the first report on the delivery of nanoconjugated DNA construct in M. rosenbergii, against nodavirus. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Fish Diseases |
| NAAS Rating: | 8.32 |
| Volume No.: | 37(9): |
| Page Number: | 1-10 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI: 10.1111/jfd.12179 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/15683 |
| Appears in Collections: | FS-CIFRI-Publication |
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