KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/17416
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Manamohan Maligeppagol*, R Manjula, Prakash M Navale, K Prasad Babu, Bhimanagoud M Kumbar and R H Laxman | en_US |
dc.date.accessioned | 2019-03-19T03:38:07Z | - |
dc.date.available | 2019-03-19T03:38:07Z | - |
dc.date.issued | 2016-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 0975-0967 (Online); 0972-5849 (Print) | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/17416 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Chilli, an important commercial crop, is recalcitrant to in vitro regeneration. In the present study, an attempt has been made to optimize the in vitro regeneration of two chilli local cultivars, viz. , G4 and LCA334, and transform G4 with a transcription factor dreb1A under the control of a desiccation inducible promoter rd29A, known to impart desiccation tolerance, using binary vector pCAMBIA 2301. Different phytohormones and their concentrations in MS medium were evaluated for in vitro response; 0.25 mg L -1 zeatin and 2 mg L -1 phenyl acetic acid (PAA) supported the highest regeneration rate (37.86%). Among the explants, cotyledonary leaf exhibited a higher (51%) regeneration response compared to that of hypocotyls (24.73%), and genotype G4 had better (33.80%) regeneration rate compared to LCA334. Rooting of the shoots was the highest in MS medium with 2 mg L -1 indole butyric acid (IBA) compared to other hormones. The presence of transgene was confirmed by polymerase chain reaction (PCR) and Southern blotting. The acclimatized transformants were grown in pots and screened for drought tolerance. Some of the transformants showed improved tolerance to drought by lower wilting compared to the control plants. The transformation and regeneration protocol described in the present study may help in optimization of transformation of chilli for agronomic traits of interest. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | NISCAIR-CSIR, India | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Transformation, chilli, drought tolerance, dreb1A | en_US |
dc.title | Genetic transformation of chilli ( Capsicum annuum L.) with Dreb 1A transcription factor known to impart drought tolerance | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Biotechnology | en_US |
dc.publication.volumeno | 15 | en_US |
dc.publication.pagenumber | 17-24 | en_US |
dc.publication.divisionUnit | Division of Biotechnology | en_US |
dc.publication.sourceUrl | http://hdl.handle.net/123456789/34473 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Horticultural Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.41 | en_US |
Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.