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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/19785
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kuntal Das, Raman Dang, Gokul Sivaraman, Rajasekharan Punathil Ellath, D. Roopa and B. Subbaiyan | en_US |
dc.date.accessioned | 2019-05-22T06:56:53Z | - |
dc.date.available | 2019-05-22T06:56:53Z | - |
dc.date.issued | 2018-10-11 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 2278-9839 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/19785 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Coscinium fenestratum (CF) (Gaertn.) Colebr. is a hard woody climber medicinal plant belongs to the family, Menispermaceae. The plant is commonly known as tree turmeric because the stem content yellow berberine, the major active constituent. The plant is widely available in Western Ghats region but became endangered due to over exploitation and very slow germination rate. Hence, the alternate in vitro tissue culture has established for reduced germination rate and to extract more amount of plant constituents from the stem callus. Full strength MS medium supplemented with 2, 4-D at 0.1 mg/l and kinetin at 2 mg/l gave callus growth in 46 days and the growth mechanism is observed first time through SEM study of callus. Thereafter, rooting of callus occurred in IBA and kinetin in combinations in half strength MS medium whereas direct rooting of stem occurred with IBA and zinc sulphate (IBA at 2 mg/l and ZnSO4 at 3 mg/l) supplemented in half strength MS media along with coconut water, within 18 days. Thereafter, extracted callus is identified with TLC, followed by estimated with HPLC and HPTLC and resulted methanol extract of CF showed higher content of berberine than aqueous extract. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Ukas | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Berberine, callus, chromatographic method, estimation, in vitro culture, organogenesis, plant hormones, ZnSO4 | en_US |
dc.title | Effect of plant hormones and zinc sulphate on rooting and callus induction in in vitro propagated Coscinium fenestratum (Gaertn.) Colebr stem and their role in estimation of secondary metabolites | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Annals of Phytomedicine | en_US |
dc.publication.volumeno | 7(1) | en_US |
dc.publication.pagenumber | 87-95 | en_US |
dc.publication.divisionUnit | Division of Plant Genetic Resources | en_US |
dc.publication.sourceUrl | 0.21276/ap.2018.7.1.10 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Horticultural Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 5.95 | en_US |
Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
File | Description | Size | Format | |
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Coscinium tissue culture-Paper.pdf | 1.04 MB | Adobe PDF | View/Open |
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