KRISHI
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/20190
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | K Kirti | en_US |
dc.contributor.author | P. Ezhil Praveena | en_US |
dc.contributor.author | T. Bhuvaneswari | en_US |
dc.contributor.author | KP. Jithendran | en_US |
dc.date.accessioned | 2019-06-12T05:09:48Z | - |
dc.date.available | 2019-06-12T05:09:48Z | - |
dc.date.issued | 2019-01-21 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/20190 | - |
dc.description | Not Available | en_US |
dc.description.abstract | This study aimed to evaluate the utility and efficiency of the Flinders Technology Associates (FTA®) Elute Card (Whatman®) as a sampling device and storage platform for RNA from Betanodavirus infected biological sample (viz.,cell culture supernatant). The retrieval of target RNA from FTA Elute Card discs with a diameter ranging from 1.2 - 2mm was found to be satisfactory for detection of Betanodavirus by reverse transcription-nested polymerase chain reaction (RT-nPCR). The viral RNA on the cards could be detected by RT-nPCR for a minimum period of 30 days of storage at 4°C, though at lower efficiency after 21 days of storage. In conclusion, FTA cards protocol provides a supplementary method for quick and easy collection of samples, preservation of RNA on a dry storage basis and detection of Betanodavirus infected fish. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Virology: Current Research | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Viral nervous necrosis | en_US |
dc.subject | Viral encephalopathy and retinopathy | en_US |
dc.subject | Betanodavirus; Diagnosis | en_US |
dc.subject | RT-nPCR | en_US |
dc.subject | FTA elute card | en_US |
dc.title | Evaluation of Flinders Technology Associates Cards as a Non-lethal Sampling Device for Molecular Diagnosis of Betanodavirus in Asian Seabass, Lates calcarifer (Bloch, 1790) | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Virology: Current Research | en_US |
dc.publication.volumeno | 3 | en_US |
dc.publication.pagenumber | 2-5 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Brackishwater Aquaculture | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | FS-CIBA-Publication |
Files in This Item:
File | Description | Size | Format | |
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VCRH-18-183.pdf | 470.57 kB | Adobe PDF | View/Open |
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