KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/22876
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sekar M. | en_US |
dc.contributor.author | Singh S.D. | en_US |
dc.contributor.author | Angel R.J. | en_US |
dc.contributor.author | Meena D.K. | en_US |
dc.contributor.author | Sivakumar N. | en_US |
dc.contributor.author | Suresh E. | en_US |
dc.contributor.author | Kathirvelpandian A | en_US |
dc.date.accessioned | 2019-09-04T04:08:21Z | - |
dc.date.available | 2019-09-04T04:08:21Z | - |
dc.date.issued | 2015-01-01 | - |
dc.identifier.citation | Sekar M., Singh S.D., Angel R.J., Meena D.K., Sivakumar N., Suresh E. and Kathirvelpandian A. 2015. Growth promoting activity of Pangasianodon hypophthalmus recombinant growth hormone expressed in Escherichia coli. Indian Journal of Fisheries, 62 (1): 70-77. | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/22876 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Recombinant growth hormone of Pangasianodon hypophthalmus (rPhGH) was efficiently expressed in Escherichia coli BL 21 (DE3) cells. The expression vector pET-32a( + ) was used to clone and express a 550 bp long cDNA fragment, which encodes the mature region of growth hormone. The rPhGH was expressed as a 6X HIS-tag fusion protein in E. coli upon induction by Isopropyl b-D-thiogalactoside, and formed insoluble inclusion bodies in the host cells. SDS-PAGE analysis indicated that the molecular weight of the fusion protein was about 23 kDa, which is comparable to the theoretical value of the mature growth hormone of the fish. The expressed protein was recovered by solubilising the inclusion bodies under denaturing conditions with urea and then the denatured proteins were refolded and purified on Ni-NTA column. The purified recombinant protein was confirmed by Western blot analysis using anti-His antibodies. Total yield of the refolded and purified protein was 20 mg l-1 of LB medium. Biological activity of the purified recombinant protein was determined in in vivo bioassay by its ability to promote growth in rohu (Labeo rohita) fingerlings, injected with three different concentrations of the hormone. A significant increase in growth was observed in rohu fingerlings administered with rPhGH at a dosage of 1.0 mg g body weight -1. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Recombinant DNA technology | en_US |
dc.title | Growth promoting activity of Pangasianodon hypophthalmus recombinant growth hormone expressed in Escherichia coli. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Fisheires | en_US |
dc.publication.volumeno | 62 (1) | en_US |
dc.publication.pagenumber | 70-77 | en_US |
dc.publication.divisionUnit | CMFRI-Visakhapatnam | en_US |
dc.publication.sourceUrl | http://eprints.cmfri.org.in/id/eprint/10351 | en_US |
dc.publication.authorAffiliation | ICAR::Central Marine Fisheries Research Institute | en_US |
dc.publication.authorAffiliation | Indian Council of Agricultural Research | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Fisheries Education | en_US |
dc.publication.authorAffiliation | ICAR::Central Inland Fisheries Research Institute | en_US |
dc.publication.authorAffiliation | ICAR::National Bureau of Fish Genetic Resources | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.29 | en_US |
Appears in Collections: | FS-CIFRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.