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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/23098
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Priyadarshi, H. | en_US |
dc.contributor.author | Alam, A., | en_US |
dc.contributor.author | Gireesh-Babu, | en_US |
dc.contributor.author | P., Das, | en_US |
dc.contributor.author | R., Kishore, | en_US |
dc.contributor.author | Kumar, S. | en_US |
dc.contributor.author | Chaudhari, A | en_US |
dc.date.accessioned | 2019-09-25T16:21:08Z | - |
dc.date.available | 2019-09-25T16:21:08Z | - |
dc.date.issued | 2012-05-01 | - |
dc.identifier.citation | Priyadarshi, H., Alam, A., Gireesh-Babu, P., Das, R., Kishore, P., Kumar, S. and Chaudhari, A. 2012. A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment. Journal of Environmental Sciences, 24(5): 963–968. | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/23098 | - |
dc.description | Not Available | en_US |
dc.description.abstract | A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of γ phage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ⩽ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | attP/attB | en_US |
dc.subject | integrationE. | en_US |
dc.subject | coli biosensorGFPmercury | en_US |
dc.title | A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg (II) in environment | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Environmental Sciences | en_US |
dc.publication.volumeno | 24(5) | en_US |
dc.publication.pagenumber | 963–968. | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/S1001-0742(11)60820-6 | en_US |
dc.publication.authorAffiliation | ICAR::Central Inland Fisheries Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 10.3 | en_US |
Appears in Collections: | FS-CIFRI-Publication |
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