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http://krishi.icar.gov.in/jspui/handle/123456789/23650
Title: | Ethanol production from sweet sorghum bagasse through process optimization using response surface methodology |
Other Titles: | Not Available |
Authors: | Lavudi Saida, Harinder Singh Oberoi, Lakshmi Narasu Mangamoori |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research ICAR-Central Institute of Post Harvest Engineering and Technology Jawahar Lal Nehru Technological University |
Published/ Complete Date: | 2017-07-08 |
Project Code: | Not Available |
Keywords: | Pichia kudriavzevii HOP-1, Response surface methodology (RSM), Simultaneous saccharification and fermentation (SSF),Separate hydrolysis and fermentation (SHF), Sweet sorghum bagasse (SSB) |
Publisher: | Springer |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In this study, comparative evaluation of acid- and alkali pretreatment of sweet sorghum bagasse (SSB) was carried out for sugar production after enzymatic hydrolysis. Results indicated that enzymatic hydrolysis of alkali-pretreated SSB resulted in higher production of glucose, xylose and arabinose, compared to the other alkali concentrations and also acid-pretreated biomass. Response Surface Methodology (RSM) was, therefore, used to optimize parameters, such as alkali concentration, temperature and time of pretreatment prior to enzymatic hydrolysis to maximize the production of sugars. The independent variables used during RSM included alkali concentration (1.5–4%), pretreatment temperature (125–140 °C) and pretreatment time (10–30 min) were investigated. Process optimization resulted in glucose and xylose concentration of 57.24 and 10.14 g/L, respectively. Subsequently, second stage optimization was conducted using RSM for optimizing parameters for enzymatic hydrolysis, which included substrate concentration (10–15%), incubation time (24–60 h), incubation temperature (40–60 °C) and Celluclast concentration (10–20 IU/g-dwt). Substrate concentration 15%, (w/v) temperature of 60 °C, Celluclast concentration of 20 IU/g-dwt and incubation time of 58 h led to a glucose concentration of 68.58 g/l. Finally, simultaneous saccharification fermentation (SSF) as well as separated hydrolysis and fermentation (SHF) was evaluated using Pichia kudriavzevii HOP-1 for production of ethanol. Significant difference in ethanol concentration was not found using either SSF or SHF; however, ethanol productivity was higher in case of SSF, compared to SHF. This study has established a platform for conducting scale-up studies using the optimized process parameters. |
Description: | Not Available |
ISSN: | 2190-572X |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biotech-3 |
NAAS Rating: | 7.8 |
Volume No.: | 7 |
Page Number: | 233 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://dx.doi.org/10.1007/s13205-017-0863-x |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/23650 |
Appears in Collections: | HS-IIHR-Publication |
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