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http://krishi.icar.gov.in/jspui/handle/123456789/26054| Title: | Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays. |
| Other Titles: | Not Available |
| Authors: | Sasi, S., Revathy, K.A. and Bhat, A. I |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
| Published/ Complete Date: | 2015-03-26 |
| Project Code: | BT/PR14813/AGR/02/761/2010 |
| Keywords: | CaMV 35 S promoter detection kanamycin sensitivity validation |
| Publisher: | Springer |
| Citation: | Sasi, S., Revathy, K.A. and Bhat, A. I. 2015. Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays. Journal of Plant Biochemistry and Biotechnology. 24: 466-469. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A loop-mediated isothermal amplification (LAMP) and real-time LAMP based assays were developed for quick and sensitive detection of transgenic black pepper plants. Primers (six each) were designed based on the nucleotide sequence of two target regions [kanamycin and Cauliflower mosaic virus (CaMV) 35 S promoter] integrated into the genome of transgenic black pepper. The following conditions proved optimal for amplification: 6 mM of magnesium sulphate, 0.4 M of betaine and 1 h of reaction time. The assay successfully detected the transgenic plants whereas no cross-reaction was recorded with non-transgenic plants. The detection limit for LAMP was up to 10000 times that for conventional PCR and 1/1000 times that for real-time LAMP. The assays were validated by testing putative transformants of black pepper. The results presented clearly showed that LAMP and real-time LAMP assays developed in this study can provide a rapid and simple approach for screening transgenic black pepper plants. |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
| NAAS Rating: | 6.77 |
| Volume No.: | 24 (4) |
| Page Number: | 466-469 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | DOI 10.1007/s13562-015-0302-1 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/26054 |
| Appears in Collections: | HS-IISR-Publication |
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