KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/26061
Title: | Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based Reverse Transcription-Quantitative PCR |
Other Titles: | Not Available |
Authors: | Siljo, A., Bhat, A.I. and Biju, C.N |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
Published/ Complete Date: | 2014-01 |
Project Code: | BT/PB/03/01/2007 |
Keywords: | Banana bract mosaic virus Cardamom Cardamom mosaic virus Detection RT-qPCR Sensitivity |
Publisher: | Springer |
Citation: | Siljo, A., Bhat, A.I. and Biju, C.N. 2014. Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based Reverse Transcription-Quantitative PCR. Virus Disease 25: 137-141. |
Series/Report no.: | Not Available; |
Abstract/Description: | Cardamom being perennial, propagated vegetatively, detecting viruses in planting material is important to check the spread of viruses through infected material. Thus development of effective and sensitive assay for detection of viruses is need of the time. In this view, assay for the detection of Cardamom mosaic virus (CdMV) and Banana bract mosaic virus (BBrMV), infecting cardamom was developed using SYBR Green one step reverse transcription- quantitative PCR (RT-qPCR). The RT-qPCR assay amplified all isolates of CdMV and BBrMV tested but no amplification was obtained with RNA of healthy plants. Recombinant plasmids carrying target virus regions corresponding to both viruses were quantified, serially diluted and used as standards in qPCR to develop standard curve to enable quantification. When tenfold serial dilutions of the total RNAs from infected plants were tested through RT-qPCR, the detection limit of the assay was estimated to be 16 copies for CdMV and 10 copies for BBrMV, which was approximately 1,000-fold higher than the conventional RT-PCR. The RT-qPCR assay was validated by testing field samples collected from different cardamom growing regions of India. This is the first report of RT-qPCR assay for the detection of CdMV and BBrMV in cardamom. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Virus Disease |
NAAS Rating: | 5.95 |
Volume No.: | 25 (1) |
Page Number: | 137-141 |
Name of the Division/Regional Station: | Crop Protection |
Source, DOI or any other URL: | DOI 10.1007/s13337-013-0170-z |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/26061 |
Appears in Collections: | HS-IISR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.