KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/28172| Title: | Development of EMA-2 recombinant antigen basedenzyme-linked immunosorbent assay for seroprevalencestudies of Theileria equi infection in Indian equine population |
| Other Titles: | Not Available |
| Authors: | Sanjay Kumar, Rajender Kumar, Ashok K. Gupta, Suresh C. Yadav,Sachin K. Goyal, Sandip K. Khurana, Raj K. Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Equines |
| Published/ Complete Date: | 2013-08-30 |
| Project Code: | Not Available |
| Keywords: | Babesia equi, Equine piroplasmosis, ELISA EMA-2, Seroprevalence, Theileria equi |
| Publisher: | Elsevier |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | tEquine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/orBabesia caballi. In the present study, we expressed a 53 kDa protein from the truncated EMA-2 gene of T. equi (Indian strain) and developedEMA-2ELISA using this expressed protein. ThisELISA is able to detect T. equi-specific antibodies in experimentally infected animals as earlyas 9 days post-infection. The assay developed was validated with the OIE recommendedcompetitive ELISA (cELISA) on 120 serum samples and significant agreement (kappa = 0.93)was observed between results of both the ELISAs which indicates suitability ofEMA-2ELISAfor use in sero-diagnosis. Diagnostic sensitivity and specificity ofEMA-2ELISA – as comparedwith cELISA – were 0.97 and 0.96, respectively. Analysis of 5651 equine serum samples– collected during 2007–2012 from 12 states of India representing eight agro-climaticzones – byEMA-2ELISA revealed 32.65% seroprevalence of T. equi in India. In conclusion, theEMA-2ELISA developed using the T. equi EMA-2 recombinant protein as antigen for detectingT. equi-specific antibodies has good diagnostic potential for sero-epidemiological surveys. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Book |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Veterinary Parasitology |
| NAAS Rating: | 8.16 |
| Volume No.: | 198 |
| Page Number: | 10-17 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | oi.org/10.1016/j.vetpar.2013.08.030 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/28172 |
| Appears in Collections: | AS-NRCE-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.