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http://krishi.icar.gov.in/jspui/handle/123456789/3175| Title: | Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers |
| Other Titles: | Not Available |
| Authors: | Ajaz Ali Bhat M .A .Haniffa M. James Milton Bilal Ahmad Paray P. R. Divya A. Gopalakrishnan |
| Published/ Complete Date: | 2014-05-28 |
| Project Code: | Not Available |
| Keywords: | Indian snakehead, Channa striatus, genetic diversity, random amplified polymorphic DNA (RAPD). |
| Publisher: | Academic Journals |
| Citation: | Bhat, A. A., Haniffa, M. A., Milton, M. J., Paray, B. A., Divya, P. R., & Gopalakrishnan, A. (2014). Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers. International Journal of Biodiversity and Conservation, 6(5), 363-372. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation among Channa striatus collected from four geographically distant locations in India. After initial PCR screening, nine random oligodecamers viz. OPA10, OPA11, OPA15, OPAC01, OPAC03, OPAC05, OPAC07, OPAC09 and OPAC19 which generated the RAPD profile for the four C. striatus populations were selected. Amplification using these nine primers resulted in fragments ranging in length between 309-3029 bp assigned to 87 loci. Estimates of Nei’s (1978) unbiased genetic distance (D) values ranged from 0.3242-0.6320. Unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed on the basis of genetic distance revealed very close genetic relationship among C. striatus populations of river Tamirabarani (Tamil Nadu) and river Periyar (Kerala). Both populations from river Tamirabarani and Periyar were found to be genetically closer to fish populations from Kolleru Lake (Andhra Pradesh) whereas C. striatus population from Brahmani River (Orissa) was found to be genetically distant from the rest of the populations. |
| Description: | Not Available |
| ISSN: | 2141-243X |
| Type(s) of content: | Research Paper |
| Sponsors: | Department of Science Technology (DST), New Delhi (SR/SO/AS-76/2007) |
| Language: | English |
| Name of Journal: | International Journal of Biodiversity and Conversation |
| Volume No.: | 6(5) |
| Page Number: | 363-372 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.5897/IJBC2013.0649 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/3175 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| article1398699985_Bhat et al.pdf | 828.3 kB | Adobe PDF | View/Open |
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