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http://krishi.icar.gov.in/jspui/handle/123456789/36855
Title: | In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiate |
Other Titles: | Not Available |
Authors: | Swati Vaish Praveen Awasth Siddharth Tiwari Shailesh Kumar Tiwari Divya Gupta Mahesh Kumar Basantani |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Vegetable Research |
Published/ Complete Date: | 2018-02-01 |
Project Code: | Not Available |
Keywords: | bioinformatics, phi and tau glutathione S-transferases, LegumeInfo, plant stress metabolism, whole-genome sequencing |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Plant glutathione S-transferases (GSTs) are integral to normal plant metabolism and biotic and abiotic stress tolerance. The GST gene family has been characterized in diverse plant species using molecular biology and bioinformatics approaches. In the current study, in silico analysis identified 44 GSTs in Vigna radiata. Of the total 44 GSTs identified, chromosomal locations of 31 GSTs were confirmed. The pI value of GST proteins ranged from 5.10 to 9.40. The predicted molecular weights ranged from 13.12 to 50 kDa. Subcellular localization analysis revealed that all GSTs were predominantly localized in the cytoplasm. The active site amino acids were confirmed to be serine in tau, phi, theta, zeta, and TCHQD; cysteine in lambda, DHAR, and omega; and tyrosine in EF1G. The gene architecture conformed to the two-exon/one-intron and three-exon/two-intron organization in the case of tau and phi classes, respectively. MEME analysis identified 10 significantly conserved motifs with the width of 8–50 amino acids. The motifs identified were either specific to a specific GST class or were shared by multiple GST classes. The results of the current study will be of potential importance in the characterization of the GST gene family in V. radiata, an economically important leguminous crop. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Genome |
NAAS Rating: | 8.04 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI: 10.1139/gen-2017-0192 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/36855 |
Appears in Collections: | HS-IIVR-Publication |
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