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http://krishi.icar.gov.in/jspui/handle/123456789/38647
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dhanshetty M, Banerjee Kaushik | en_US |
dc.date.accessioned | 2020-07-31T06:23:38Z | - |
dc.date.available | 2020-07-31T06:23:38Z | - |
dc.date.issued | 2019-11-01 | - |
dc.identifier.citation | Dhanshetty M, Banerjee Kaushik (2019). Simultaneous direct analysis of aflatoxins and ochratoxin A in cereals and their processed products by ultra-high performance liquid chromatography with fluorescence detection. Journal of AOAC International 102 (6): 1666-1672. DOI: https://doi.org/10.1093/jaoac/102.6.1666 | en_US |
dc.identifier.uri | https://doi.org/10.1093/jaoac/102.6.1666 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/38647 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Background: Mycotoxins such as aflatoxins (AFs) and ochratoxin A (OTA) can pose severe health hazards because of their toxicity. Given a wide range of food matrices susceptible to fungal infections and possible cooccurrence of mycotoxins at different concentrations, validated multimycotoxin and multimatrix methods are strongly warranted. Objective: The aim of this research was to develop a simple and fast ultra-high performance LC (UHPLC) fluorescence detection (FLD)–based method to simultaneously determine AFs (B1, G1, B2, and G2) and OTA and, furthermore, to carry out single-laboratory validation in a range of cereals and processed product matrices. Methods: The sample preparation involved homogenization and extraction with methanol–water (80 + 20). For cleanup, an aliquot (3 mL) was diluted with phosphate-buffered saline, loaded on an immunoaffinity column (AFLAOCHRA PREP®), and eluted with methanol (1 mL). The cleaned extract was diluted with 0.2% acetic acid (at a 1:1 ratio) before injection into an ultra-high performance liquid chromatograph. To perform simultaneous analysis of AFs and OTA, the FLD program was developed by switching the excitation wavelength in a single chromatographic run. Results: The method provided LOQs of 0.25 and 1 ng/g for AFs and OTA, respectively, without involving any derivatization. In rice, the recoveries of AFs ranged from 84 to 106%, whereas OTA had a recovery above 72%, with the repeatability relative SDs <12% for both analytes. The method was successfully applied to a range of naturally contaminated market samples. Conclusions: The method is suitable for regulatory testing because of its significant time and cost effectiveness and sensitivity in compliance with the regulatory maximum levels. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Oxford University Press | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Aflatoxins | en_US |
dc.subject | Ochratoxin A | en_US |
dc.subject | Cereals | en_US |
dc.subject | Processed products | en_US |
dc.subject | Ultra-High Performance Liquid Chromatography with Fluorescence Detection | en_US |
dc.title | Simultaneous direct analysis of aflatoxins and ochratoxin A in cereals and their processed products by ultra-high performance liquid chromatography with fluorescence detection | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of AOAC International | en_US |
dc.publication.volumeno | 102 | en_US |
dc.publication.pagenumber | 1666–1672 | en_US |
dc.publication.divisionUnit | National Reference Laboratory | en_US |
dc.publication.sourceUrl | https://doi.org/10.1093/jaoac/102.6.1666 | en_US |
dc.publication.authorAffiliation | ICAR::National Research Centre for Grapes | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | HS-NRCG-Publication |
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