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Title: | High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell |
Other Titles: | Not Available |
Authors: | Oulkar D, Goon A., Dhanshetty M., Khan Z., Satav S., Banerjee Kaushik |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre for Grapes |
Published/ Complete Date: | 2017-12-26 |
Project Code: | Not Available |
Keywords: | Aflatoxin analysis Aflatoxins in peanuts and cereals UPLC-fluorescence detection |
Publisher: | Taylor & Francis |
Citation: | Oulkar D, Goon A., Dhanshetty M., Khan Z., Satav S., Banerjee Kaushik (2017). High-sensitivity direct analysis of aflatoxins in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell. Journal of Environmental Science and Health, Part B 53 (4): 255-260 |
Series/Report no.: | Not Available; |
Abstract/Description: | This paper reports a sensitive and cost effective method of analysis for aflatoxins B1, B2, G1 and G2. The sample preparation method was primarily optimised in peanuts, followed by its validation in a range of peanut-processed products and cereal (rice, corn, millets) matrices. Peanut slurry [12.5 g peanut + 12.5 mL water] was extracted with methanol: water (8:2, 100 mL), cleaned through an immunoaffinity column and thereafter measured directly by ultra-performance liquid chromatography-fluorescence (UPLC-FLD) detection, within a chromatographic runtime of 5 minutes. The use of a large volume flow cell in the FLD nullified the requirement of any post-column derivatisation and provided the lowest ever reported limits of quantification of 0.025 for B1 and G1 and 0.01 μg/kg for B2 and G2. The single laboratory validation of the method provided acceptable selectivity, linearity, recovery and precision for reliable quantifications in all the test matrices as well as demonstrated compliance with the EC 401/2006 guidelines for analytical quality control of aflatoxins in foodstuffs. |
Description: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was |
NAAS Rating: | 7.7 |
Volume No.: | 53 |
Page Number: | 255-260 |
Name of the Division/Regional Station: | National Reference Laboratory |
Source, DOI or any other URL: | https://doi.org/10.1080/03601234.2017.1410416 |
URI: | https://doi.org/10.1080/03601234.2017.1410416 http://krishi.icar.gov.in/jspui/handle/123456789/38656 |
Appears in Collections: | HS-NRCG-Publication |
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