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http://krishi.icar.gov.in/jspui/handle/123456789/41639
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dimpal Thakuria | en_US |
dc.contributor.author | Neetu Shahi | en_US |
dc.contributor.author | Atul Kumar Singh | en_US |
dc.contributor.author | Victoria Chanu Khangembam | en_US |
dc.contributor.author | Arvind Kumar Singh | en_US |
dc.contributor.author | Satish Kumar | en_US |
dc.date.accessioned | 2020-09-26T10:11:06Z | - |
dc.date.available | 2020-09-26T10:11:06Z | - |
dc.date.issued | 2017-01-01 | - |
dc.identifier.citation | Thakuria, D., Shahi, N., Singh, A.K., Chanu, K.V., Singh, A.K. and Kumar, S. (2017). Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments. PLoS ONE, 12(10): e0185892. https://doi.org/10.1371/journal.pone.0185892. | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/41639 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Kisspeptin 1 is a neuropeptide hormone of the RFamide family, which act as an upstream regulator of brain-pituitary-gonad (BPG) axis in most vertebrates including teleosts. In the present study, a 16 amino acid long putative mature bioactive peptide (kiss 1) from preprokisspeptin 1 of golden mahseer, Tor putitora (Hamilton, 1822), was synthesized and characterized using an integrated (experimental and in silico) approach. The far-UV circular dichroism (CD) spectrum of this peptide was evaluated both in aqueous and membrane mimicking solvents (TFE, HFIP and Dioxane). The results indicate that kiss 1 peptide adopted helical, turn and β conformations in membrane like environments. The near-UV CD spectroscopy was also carried out to examine the tertiary packing around aromatic residues of kiss 1 peptide and the peptide-membrane complex. The kiss 1 peptide exhibited little signal in water, but a prominent negative band was observed at around 275 nm when membrane mimetic solution was added. The observed ordered conformations of kiss 1 peptide in the different solvents indicated its potential biological activity which could enhance the secretion of gonadotropin-releasing hormone (GnRH) at BPG axis. The conformational information generated from the present study reinforces the application prospects of bioactive synthetic peptide analogs of kisspeptin 1 in improving the reproductive performances of important cultivable fish species. | en_US |
dc.description.sponsorship | Indian Council of Agricultural Research (ICAR) | en_US |
dc.language.iso | English | en_US |
dc.publisher | PLOS / Public Library of Science | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | NA | en_US |
dc.title | Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | PLOS One | en_US |
dc.publication.volumeno | 12(10) | en_US |
dc.publication.pagenumber | e0185892 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1371/journal.pone.0185892 | en_US |
dc.publication.authorAffiliation | ICAR::Directorate of Cold Water Fisheries Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 8.74 | en_US |
Appears in Collections: | FS-DCWFR-Publication |
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