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http://krishi.icar.gov.in/jspui/handle/123456789/45143
Title: | Identification of simple sequence repeat markers tightly linked to lipoxygenase-2 gene in soybean. |
Other Titles: | Not Available |
Authors: | Vineet Kumar, Anita Rani, Reena Rawal |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Directorate of Soybean Research |
Published/ Complete Date: | 2014-10-01 |
Project Code: | Not Available |
Keywords: | Lipoxygenase 2, Lox 2 locus, off-flavour, soybean, SSR markers |
Publisher: | NISCAIR-CSIR, India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Lipoxygenase-2, controlled by single dominant gene, is prime cause of off-flavour generated in soy products. The objective of the present investigation was to validate the SSR markers (Sat_074 & Satt522) reported to be linked to Lox2 locus and to identify tightly linked SSR markers in F2 mapping population 1 and population 2, generated from crosses between Indian soybean varieties JS97-52 and JS93-05 as the conventional parents (Lox2Lox2) and PI596540 as the source of null allele of lipoxygenase-2 (lox2lox2), respectively. Parental polymorphism was surveyed using SSR markers Sat_074, Satt522 and 7 SSR markers in the neighbouring genomic region of Lox2 locus. F2:3 seeds from each F2 individual in both the mapping populations were phenotyped for the presence/absence of lipoxygenase-2 isozyme. The SSR marker Sat_074 was not found to be polymorphic for the parental combination JS97-52 x PI596540. Satt522 was found to be at 9.3 and 13.5 cM from Lox2 locus in mapping population 1 and 2, respectively. We found two additional new SSR markers Sat_417 and Satt656 closely linked to Lox2 locus. SSR marker Sat_417 was found to be at a close distance of 6.9 and 6.6 cM from Lox2 locus; while Satt656 was identified to be tightly linked to Lox2 locus at a distance of 2.7 and 2.1 cM in the mapping population 1 and 2, respectively. Thus, the SSR marker Satt656 can be employed in marker assisted selection for transferring null allele (lox2) of the lipoxygenase-2 in the background of popular Indian soybean cultivars. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Biotechnology |
NAAS Rating: | 6.41 |
Volume No.: | 13(4) |
Page Number: | 455-458 |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/45143 |
Appears in Collections: | CS-DSBR-Publication |
Files in This Item:
File | Description | Size | Format | |
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IJBT 13(4) 455-458.pdf | 107.22 kB | Adobe PDF | View/Open |
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