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http://krishi.icar.gov.in/jspui/handle/123456789/4681
Title: | Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus notopterus (Pallas, 1769). |
Other Titles: | Not Available |
Authors: | Arti Gupta Kuldeep K. Lal Peyush Punia Rajeev K. Singh Vindhya Mohindra Rama S. Sah Rajesh Kumar Rupesh K. Luhariya Arvind K. Dwivedi Prachi Masih R. M. Mishra J. K. Jena |
Published/ Complete Date: | 2013-09-26 |
Project Code: | Not Available |
Keywords: | Notopterus notopterus Microsatellite markers Polymorphism Genetic diversity |
Publisher: | SPRINGER LINK |
Citation: | Gupta A, Lal KK, Punia P, Singh RK, Mohindra V, Sah RS, Kumar R, Luhariya RK, Dwivedi AK, Masih P, Mishra RM, Jena JK. Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus notopterus (Pallas, 1769). Mol Biol Rep. 2013 Dec;40(12):6625-31. doi: 10.1007/s11033-013-2776-z. Epub 2013 Sep 26. PubMed PMID: 24072656. |
Series/Report no.: | Not Available; |
Abstract/Description: | Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. notopterus. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Molecular Biology Reports |
NAAS Rating: | 7.4 |
Volume No.: | 40(12) |
Page Number: | 6625-6631 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s11033-013-2776-z |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4681 |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
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art%3A10.1007%2Fs11033-013-2776-z.pdf | 204.86 kB | Adobe PDF | View/Open |
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