KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4700
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Akanksha Singh | en_US |
dc.contributor.author | A. S. Barman | en_US |
dc.contributor.author | Neeraj Sood | en_US |
dc.contributor.author | Vindhya Mohindra | en_US |
dc.date.accessioned | 2017-07-17T06:57:45Z | - |
dc.date.available | 2017-07-17T06:57:45Z | - |
dc.date.issued | 2013-10-27 | - |
dc.identifier.citation | Singh A, Barman AS, Sood N, Mohindra V. Transcriptome generation and analysis from spleen of Indian catfish, Clarias batrachus (Linnaeus, 1758) through normalized cDNA library. Mol Biol Rep. 2013 Dec;40(12):6965-75. doi: 10.1007/s11033-013-2816-8. PubMed PMID: 24162781. | en_US |
dc.identifier.other | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/4700 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Catfishes are commercially important fish for both the fisheries and aquaculture industry. Clarias batrachus, an Indian catfish species is economically important owing to its high demand. A normalized cDNA library was constructed from spleen of the Indian catfish to identify genes associated with immune function. One thousand nine hundred thirty seven ESTs were submitted to the GenBank with an average read length of approximately 700 bp. Clustering analysis of ESTs yielded 1,698 unique sequences, including 184 contigs and 1,514 singletons. Significant homology to known genes was found by homology searches against data in GenBank in 576 (34 %) ESTs, including similarity to functionally annotated unigenes for 158 ESTs. Additionally, 433 ESTs revealed similarity to unigenes and ESTs in the dbEST but the remaining 658 EST sequences (39 %) did not match any sequence in GenBank. Of a total of 1,698 ESTs generated, 65 ESTs were found to be associated with immune functions. Gene Ontology and KEGG pathway analyses of C. batrachus ESTs collectively revealed a preponderance of immune relevant pathways apart from the presence of pathways involved in protein processing, localization, folding and protein degradation. This study constitutes first EST analysis of lymphoid organ in aquaculturally important Indian catfish species and could pave the way for further research of immune-related genes and functional genomics in this catfish. | en_US |
dc.description.sponsorship | Department of Biotechnology (DBT), Government of India | en_US |
dc.language.iso | English | en_US |
dc.publisher | SPRINGER LINK | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Indian catfish | en_US |
dc.subject | Clarias batrachus | en_US |
dc.subject | Expressed sequence tags | en_US |
dc.subject | cDNA library | en_US |
dc.subject | Normalisation | en_US |
dc.subject | Functional annotation | en_US |
dc.title | Transcriptome generation and analysis from spleen of Indian catfish, Clarias batrachus (Linnaeus, 1758) through normalized cDNA library | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Molecular Biology Reports | en_US |
dc.publication.volumeno | 40(12) | en_US |
dc.publication.pagenumber | 6965-6975 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.1007/s11033-013-2816-8 | en_US |
dc.publication.naasrating | 7.4 | en_US |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art%3A10.1007%2Fs11033-013-2816-8.pdf | 478.33 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.