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http://krishi.icar.gov.in/jspui/handle/123456789/47576| Title: | Characterization of STP4 promoter in Indian mustard Brassica juncea for use as an aphid responsive promoter |
| Other Titles: | Not Available |
| Authors: | Chet Ram Murali Krishna Koramutla Rekha Kansal Ajay Arora Pradeep K. Jain Ramcharan Bhattacharya |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute for Arid Horticulture ICAR::National Institute for Plant Biotechnology |
| Published/ Complete Date: | 2020-07-16 |
| Project Code: | Not Available |
| Keywords: | Aphid-responsive genes Plant inducible promoter Promoter analysis Brassica juncea Agroinfiltration Transgenics |
| Publisher: | Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Objective Brassica juncea, a major oilseed crop, suffers substantial yield losses due to infestation by mustard aphids (Lipaphis erysimi). Unavailability of resistance genes within the accessible gene pool underpins significance of the transgenic strategy in developing aphid resistance. In this study, we aimed for the identification of an aphid-responsive promoter from B. juncea, based on the available genomic resources. Results A monosaccharide transporter gene, STP4 in B. juncea was activated by aphids and sustained increased expression as the aphids colonized the plants. We cloned the upstream intergenic region of STP4 and validated its stand-alone aphid-responsive promoter activity. Further, deletion analysis identified the putative cis-elements important for the aphid responsive promoter activity. Conclusion The identified STP4 promoter can potentially be used for driving high level aphidinducible expression of transgenes in plants. Use of aphid-responsive promoter instead of constitutive promoters can potentially reduce the metabolic burden of transgene-expression on the host plant. |
| Description: | Not Available |
| ISSN: | 0141-5492 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Biotechnology Letters |
| NAAS Rating: | 7.98 |
| Impact Factor: | 1.997 |
| Volume No.: | 42 |
| Page Number: | 2013–2033 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s10529-020-02961-7(0123456789().,-volV() 0123458697().,-volV) |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/47576 |
| Appears in Collections: | HS-CIAH-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 14. Characterization of STP4 promoter in Indian mustard.pdf | 4.88 MB | Adobe PDF | View/Open |
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