KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/48786
Title: | [PDF] from isrc.in Endosymbiotic Bacteria Associated with Aphis gossypii Glover (Hemiptera: Aphidae) infesting taro |
Other Titles: | Not Available |
Authors: | BG Sangeetha, EV Sreejitha, CA Jayaprakas, ER Harish |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Central Tuber Crops Research Institute |
Published/ Complete Date: | 2019-12-31 |
Project Code: | Not Available |
Keywords: | Endosymbiotic bacteria, aphids, 16S rDNA, molecular characterization, Bacillus |
Publisher: | Indian Society for Root Crops |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Aphids are one of the important sucking pests of many agriculturally important crops, associated with a wide variety of bacterial endosymbionts that confer many ecologically relevant traits to the host insect. Endosymbiotic bacteria (ESB) play a vital role even in the physiology of the host, hence identification of ESB associated with the aphids will help to develop important strategies for the management of this noxious pest. In the present study molecular characterization of ESB associated with the aphid, Aphis gossypii infesting taro was done. Morphological characters of the four strains revealed that each isolate has different colony characters. Further, the genomic DNA was isolated from each of the EPB isolates and PCR amplification of 16S rRNA gene was carried out using universal primers. The 16S rDNA gene sequences of endosymbiotic bacterial isolates were generated by sequencing the PCR product and were aligned with each other by using BioEDIT software. The nucleotide sequences were compared with those in the NCBI databases using the Basic Local Alignment Search and four genus viz., Bacillus, Pesudomonas, Pantoea and Staphylococcus were confirmed. From the aligned sequences phylogenetic tree was constructed by the Neighbor-Joining method using MEGA version X. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | ICAR |
Language: | English |
Name of Journal: | Journal of Root Crops |
Volume No.: | 45 |
Page Number: | 41-46 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | ISSN 2454-9053 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/48786 |
Appears in Collections: | HS-CTCRI-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
553-1713-1-PB.pdf | 1.39 MB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.