Genetic diversity of Indian major carp, Labeo calbasu (Hamilton, 1822) populations inferred from microsatellite loci

Abstract

Genetic variation was assessed in 367 samples of Labeo calbasu from twelve riverine locations across India using nine polymorphic microsatellite loci. The mean value of the observed heterozygosity (Hobs.) after correction for null allele ranged from 0.50 (loci R3* and Lr38* in Satluj) to 1.00 (Lr29* in Bhagirathi) whereas mean number of alleles per locus ranged from 4.66 (Narmada) to 10.11 (Ghaghra). The genetic data corrected for possible null alleles, analyzed with score test did not reveal significant deviation from HW equilibrium at any locus in any population after probability level was adjusted for Bonferroni corrections. Pair wise Fst and allelic homogeneity tests indicated distinct population structure in wild L. calbasu. Fst for all the samples combined over all loci was found to be 0.035 suggesting that 3.5% of the total variation was due to genetic differentiation. Analysis of Molecular Variance (AMOVA) indicated that most of the genetic variability was within populations from the same geographical locations.