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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4986
Title: | Gene Cloning, Expression, and Characterization of Recombinant Aerolysin from Aeromonas hydrophila |
Other Titles: | Not Available |
Authors: | Vijai Singh Pallavi Somvanshi Gaurav Rathore D. Kapoor B. N. Mishra |
Published/ Complete Date: | 2010-04 |
Project Code: | Not Available |
Keywords: | Aeromonas hydrophila Aerolysin Expression RBCs Vaccine |
Publisher: | SPRINGER |
Citation: | Singh, V., Somvanshi, P., Rathore, G., Kapoor, D. and Mishra, B.N., 2010. Gene cloning, expression, and characterization of recombinant aerolysin from Aeromonas hydrophila. Applied biochemistry and biotechnology, 160(7), pp.1985-1991. |
Series/Report no.: | Not Available; |
Abstract/Description: | Aerolysin is a significant virulent toxin protein secreted by Aeromonas hydrophila; it produces deep wound infections and hemorrhagic septicemia. The complete aerolysin gene (1,482 bp) was amplified from A. hydrophila. Furthermore, it was cloned and expressed into Escherichia coli BL21(DE3) codon plus RP cells using 0.5 mM IPTG for induction. The protein size was 54 kDa as estimated by SDS-PAGE, and it was purified by Ni–NTA affinity chromatography. Anti-His antibodies were used to characterize the expressed aerolysin by Western blotting and showed hemolytic activity with fish red blood cells. Aerolysin may be used as immunoassays for earlier control of A. hydrophila and is also compatible for vaccination. |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Applied Biochemistry and Biotechnology |
NAAS Rating: | 8.28 |
Volume No.: | 160 |
Page Number: | 1985–1991. |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s12010-009-8752-3 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4986 |
Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
File | Description | Size | Format | |
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art%3A10.1007%2Fs12010-009-8752-3.pdf | 161.71 kB | Adobe PDF | View/Open |
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