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http://krishi.icar.gov.in/jspui/handle/123456789/50613| Title: | Development of caudal fin cell line from hill trout Barilius bendelisis (Hamilton, 1807) for cytotoxicity and transfection studies |
| Other Titles: | Not Available |
| Authors: | Murali Sanjeev Kumar Pankaj Soni Ravindra Kumar Neha Singh Shreya Srivastava Akhilesh Kumar Mishra Vijay Kumar Singh Basdeo Kushwaha. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources, Lucknow |
| Published/ Complete Date: | 2020-10-14 |
| Project Code: | Not Available |
| Keywords: | Barilius bendelisis Cell line Heavy metal cytotoxicity NRFC |
| Publisher: | University of Zagreb Faculty of Agriculture Department of Fisheries, Beekeeping, Game Management and Special Zoology |
| Citation: | Kumar, M. S., Soni, P., Kumar, R., Singh, N., Srivastava, S., Mishra, A. K., Singh, V. K., Kushwaha, B. (2021): Development of caudal fin cell line from hill trout Barilius bendelisis (Hamilton, 1807) for cytotoxicity and transfection studies. Croatian Journal of Fisheries, 79, 15-24. DOI: 10.2478/ cjf-2021-0002. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/ pollutant and gene expression. |
| Description: | Not Available |
| ISSN: | 1330-061X |
| Type(s) of content: | Research Paper |
| Sponsors: | Department of Biotechnology, Government of India, New Delhi, India |
| Language: | English |
| Name of Journal: | Croatian Journal of Fisheries |
| Journal Type: | The Croatian Journal of Fisheries publishes papers that deal with both freshwater and marine fisheries. Areas covered by the Journal include ichthyology, aquaculture, ecology and diseases of fish and other aquatic organisms, problems of open waters and other topics related to the fisheries field |
| Impact Factor: | 0.8 |
| Volume No.: | 79(1) |
| Page Number: | 15-24 |
| Name of the Division/Regional Station: | Molecular Biology and Biotechnology Division |
| Source, DOI or any other URL: | 10.2478/cjf-2021-0002 https://sciendo.com/article/10.2478/cjf-2021-0002 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50613 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Ribarstvo_79_1_2021_RAD_2.pdf | 1.43 MB | Adobe PDF | View/Open |
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