KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50632Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kanupriya Chaturvedi | en_US |
| dc.contributor.author | Manamohan, M | en_US |
| dc.contributor.author | Leela Sahijram | en_US |
| dc.date.accessioned | 2021-07-28T08:10:54Z | - |
| dc.date.available | 2021-07-28T08:10:54Z | - |
| dc.date.issued | 2019-10-01 | - |
| dc.identifier.citation | Not Available | en_US |
| dc.identifier.issn | Not Available | - |
| dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/50632 | - |
| dc.description | Not Available | en_US |
| dc.description.abstract | The purpose of this study was to investigate the effect of various DNA methylation modifying agents on genome wide global DNA methylation status and to develop papaya cv. Surya plants with useful and stable horticulturally important variations. DNA methylation-status modifying agents (Zebularine), 5-azacytidine, 5-aza deoxycytidine (DNA hypomethylating agents), 3’-azido-deoxythymidine (DNA hypermethylating agent) were used at two concentrations (0.3mM and 0.5mM) on papaya cv. Surya zygotic embryos for incubation periods ranging from 0-120 hrs. The technique of MS-RAPD-PCR which resolves global DNA methylation pattern was employed. A total of 87 primers were tested on the control samples, 55 primers showed PCR amplification, of which 11 showed polymorphic bands. Six primers showing clear and reproducible bands were selected further for analysing methylation-modifying-agent treated samples. This technique was found to be reliable and efficient for detecting changes in DNA methylation at both the scale and pattern in a genome wide standpoint. | en_US |
| dc.description.sponsorship | Not Available | en_US |
| dc.language.iso | English | en_US |
| dc.publisher | HSI | en_US |
| dc.subject | Carica papaya, MS-RAPD-PCR, zygotic embryo | en_US |
| dc.title | Modifying DNA methylation pattern in papaya embryos to harness useful and stable variants. | en_US |
| dc.title.alternative | Not Available | en_US |
| dc.type | Research Paper | en_US |
| dc.publication.projectcode | Not Available | en_US |
| dc.publication.journalname | Indian Journal of Horticulture | en_US |
| dc.publication.volumeno | 76 (3) | en_US |
| dc.publication.pagenumber | 522-527 | en_US |
| dc.publication.divisionUnit | Fruit Crops | en_US |
| dc.publication.sourceUrl | 10.5958/0974-0112.2019.00082.3 | en_US |
| dc.publication.authorAffiliation | ICAR::Indian Institute of Horticultural Research | en_US |
| dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
| dc.publication.naasrating | 6.16 | en_US |
| Appears in Collections: | HS-IIHR-Publication | |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.