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http://krishi.icar.gov.in/jspui/handle/123456789/50777| Title: | Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes |
| Other Titles: | Not Available |
| Authors: | Ravindra Kumar Vishwamitra Singh Baisvar Basdeo Kushwaha S Murali Vijay Kumar Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources, Lucknow |
| Published/ Complete Date: | 2020-05-02 |
| Project Code: | Not Available |
| Keywords: | BAC clones BAC DNA BAC end sequencing · Clarias magur · FISH Clarias magur FISH |
| Publisher: | Springer |
| Citation: | Kumar, R., Baisvar, V.S., Kushwaha, B., Murali, S. and Singh, V.K., 2020. Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes. Molecular biology reports, 47(3), pp.2405-2413. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Bacterial artificial chromosome (BAC) library is an important genomic resource useful in targeted marker development, positional cloning, physical mapping and a substrate for genome sequencing for better understanding the genome organization of a species. The present manuscript elucidates the improvement in protocols for economical and efficient BAC insert DNA isolation, BAC end sequencing and FISH for physical localization on the metaphase chromosome complements. BAC clones of Clarias magur, maintained in 384—well plate format in our laboratory, were used in this study. The protocols gave consistent and efficient results. We use routinely these protocols for BAC insert DNA extraction, generating end sequence data of the clone and constructing DNA probes to hybridize on the metaphase spreads of C. magur using FISH for physical their localization. |
| Description: | Not Available |
| ISSN: | 1573-4978 (web) 0301-4851 (print) |
| Type(s) of content: | Research Paper |
| Sponsors: | CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi, |
| Language: | English |
| Name of Journal: | Molecular Biology Reports |
| Journal Type: | Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews. |
| NAAS Rating: | 7.4 |
| Impact Factor: | 2.316 |
| Volume No.: | 47 (3) |
| Page Number: | 2405–2413 |
| Name of the Division/Regional Station: | Molecular Biology and Biotechnology |
| Source, DOI or any other URL: | 10.1007/s11033-020-05283-z https://link.springer.com/article/10.1007/s11033-020-05283-z |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50777 |
| Appears in Collections: | FS-NBFGR-Publication |
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