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http://krishi.icar.gov.in/jspui/handle/123456789/50783| Title: | Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita |
| Other Titles: | Not Available |
| Authors: | Iliyas Rashid Vishwamitra Singh Baisvar Mahender Singh Ravindra Kumar Prachi Srivastava Basdeo Kushwaha Ajey Kumar Pathak |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources, Lucknow AMITY University, Uttar Pradesh, Lucknow Campus, Lucknow |
| Published/ Complete Date: | 2020-05-01 |
| Project Code: | Not Available |
| Keywords: | HMOX1 Hypoxia Labeo rohita Phylogenetics SSR |
| Publisher: | Academic Press |
| Citation: | Rashid, I., Baisvar, V.S., Singh, M., Kumar, R., Srivastava, P., Kushwaha, B. and Pathak, A.K., 2020. Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita. Genomics, 112(3), pp.2327-2333. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The HMOX1 gene plays role in several biological processes and is also responsive to hypoxia stress. Freshwater carp fish, Labeo rohita, is reported as hypoxia sensitive, but the information of annotated hypoxia genes in public domain is very scanty for this species. Here, an attempt was made to isolate and characterize HMOX1 gene in L. rohita using information from zebrafish. HMOX1 gene was obtained by mapping HMOX1 protein of zebrafish over assembled genome of L. rohita. Aligned region was used for designing primers for HMOX1 amplification. Eight overlapping sets of primers were designed for amplifying ~540 bp long successive overlapping fragments. Splicing of overlapping amplicons generated 3715 bp fragment that was confirmed as HMOX1 gene having full coding region with 6 exons between 184 and 2156 bp positions. HMOX1 characterization is an initiative for L. rohita genes annotation to support the characterization of new genes in the important species. |
| Description: | Not Available |
| ISSN: | 0888-7543 (print) 1089-8646 (web) |
| Type(s) of content: | Research Paper |
| Sponsors: | CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi, |
| Language: | English |
| Name of Journal: | Genomics |
| Journal Type: | Genomics is a research journal which publishes articles describing development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Aming to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope |
| NAAS Rating: | 12.21 |
| Impact Factor: | 6.205 |
| Volume No.: | 112 (3) |
| Page Number: | 2327-2333. |
| Name of the Division/Regional Station: | Molecular Biology and Biotechnology |
| Source, DOI or any other URL: | 10.1016/j.ygeno.2020.01.004 https://www.sciencedirect.com/science/article/pii/S0888754318305974 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50783 |
| Appears in Collections: | FS-NBFGR-Publication |
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