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  2. Fisheries A6
  3. ICAR-National Bureau of Fish Genetic Resources I9
  4. FS-NBFGR-Publication
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Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/5085
Title: Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis
Other Titles: Not Available
Authors: Daoud Ali
Naresh Sahebrao Nagpure
Sudhir Kumar
Ravindra Kumar
Basdeo Kushwaha
Published/ Complete Date: 2008-05
Project Code: Not Available
Keywords: Channa punctatus
Chlorpyrifos
Comet assay
Genotoxicity
Micronucleus assay
Publisher: Elsevier
Citation: Ali D, Nagpure NS, Kumar S, Kumar R, Kushwaha B. Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Chemosphere. 2008 May;71(10):1823-31. doi: 10.1016/j.chemosphere.2008.02.007. Epub 2008 Mar 24. PubMed PMID: 18359502.
Series/Report no.: Not Available;
Abstract/Description: Chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridylphosphorothioate) is one of the organophosphate pesticides widely used in agricultural practices throughout world and irreversible inhibitor of cholinesterase in all animal species. Limited efforts have been made to study acute genotoxic effects of chlorpyrifos (CPF) in different tissues of fish using genotoxic biomarkers. Therefore, the present investigation was aimed to study the induction of DNA damage by CPF in freshwater teleost fish Channapunctatus using micronucleus assay (MN assay) and alkaline single-cell gel electrophoresis (comet assay). The value of LC(50) - 96 h of CPF was determined as 811.98 microgl(-1) for C. punctatus, in a semi-static system and on the basis of LC(50) value three acute concentrations viz., 203, 406 and 609 microgl(-1) were determined. The fishes were exposed to the different concentrations of CPF for 96 h and samplings were done at regular intervals for assessment of the MN frequencies and DNA damage. In general, significant effects (P<0.01) from both concentrations and time of exposure were observed in exposed fishes. It was found that the micronucleus induction was highest on 96 h at all concentrations in the peripheral blood. Similar trend was observed for the DNA damage measured in terms of the percentage of tail DNA in the lymphocyte and gill cells. This study explored the combined use of micronucleus assay and comet assay for in vivo laboratory studies using fresh water fish for screening the genotoxic potential of xenobiotics.
Description: Not Available
Type(s) of content: Research Paper
Sponsors: Not Available
Language: English
Name of Journal: Chemosphere
NAAS Rating: 11.78
Volume No.: 71(10)
Page Number: 1823-1831
Name of the Division/Regional Station: Not Available
Source, DOI or any other URL: 10.1016/j.chemosphere.2008.02.007
URI: http://krishi.icar.gov.in/jspui/handle/123456789/5085
Appears in Collections:FS-NBFGR-Publication

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