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http://krishi.icar.gov.in/jspui/handle/123456789/50987
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | R Kumar | en_US |
dc.contributor.author | C Ravi | en_US |
dc.contributor.author | S Das | en_US |
dc.contributor.author | A Dharmaratnam | en_US |
dc.contributor.author | V S Basheer | en_US |
dc.contributor.author | T R Swaminathan | en_US |
dc.date.accessioned | 2021-07-29T05:49:45Z | - |
dc.date.available | 2021-07-29T05:49:45Z | - |
dc.date.issued | 2018-07-26 | - |
dc.identifier.citation | Kumar, R., Ravi, C., Das, S. et al. Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar, Astronotus ocellatus. Fish Physiol Biochem 45, 123–131 (2019). https://doi.org/10.1007/s10695-018-0542-9 | en_US |
dc.identifier.issn | 1573-5168 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/50987 | - |
dc.description.abstract | Astronotus ocellatus, commonly called the oscar, is one of the popular cichlids among aquarium hobby. The present study deals with the development and characterization of a new cell line from caudal fin of A. ocellatus. The cell line was cultured in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 °C. The optimum temperature and FBS concentration for cell growth were tested with temperature ranges from 20 to 37 °C and FBS concentrations of 5-20% at 28 °C. The Astronotus ocellatus fin cell line has been subcultured 45 times since its development and the modal chromosome number (2n) is 48. The cell line is composed mainly of epithelial cells as confirmed by immunocytological technique using anti-cytokeratin antibodies. The cell line was cryopreserved at different passage levels and the revival efficiency showed 80% survival rate. Partial sequence amplification and sequencing of two genes, mitochondrial 16S ribosomal RNA and cytochrome oxidase I, confirmed the origin of cell line. The cell line did not show Mycoplasma contamination. The cells showed good transfection efficiency when transfected with 2 μg of pAcGFP1-N1 expression vector. The extracellular products of fish bacterial pathogens viz., Aeromonas hydrophila and A. caviae, were cytotoxic to AOF cells but were not susceptible to Cyprinid herpes virus 2. The development of AOF cell line will have significant applications in fish virology and will prove useful to isolate pathogens in the event of sudden viral disease outbreak and for the development of vaccines and diagnostic kits. | en_US |
dc.description.sponsorship | National Fisheries Development Board, Department of Animal Husbandry and Dairying, Govt. of India (G/Nat. Surveillance/2013 dated 16.08.2013), for the funding | en_US |
dc.language.iso | English | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | AOF | en_US |
dc.subject | Astronotus ocellatus | en_US |
dc.subject | Cell line | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Fish virus | en_US |
dc.subject | Oscar fish | en_US |
dc.title | Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar, Astronotus ocellatus | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Fish Physiology and Biochemistry | en_US |
dc.publication.volumeno | 45 (1) | en_US |
dc.publication.pagenumber | 123-131 | en_US |
dc.publication.divisionUnit | Peninsular and Marine Fish Genetic Resources Centre, Kochi | en_US |
dc.publication.sourceUrl | 10.1007/s10695-018-0542-9 | en_US |
dc.publication.sourceUrl | https://link.springer.com/article/10.1007%2Fs10695-018-0542-9 | en_US |
dc.publication.authorAffiliation | ICAR::National Bureau of Fish Genetic Resources, Lucknow | en_US |
dc.publication.authorAffiliation | Peninsular and Marine Fish Genetic Resources Centre, ICAR-National Bureau of Fish Genetic Resources, CMFRI Campus, Kochi, | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | An international journal publishing original research papers and reviews on all physiological aspects of fishes | en_US |
dc.publication.naasrating | 8.24 | en_US |
dc.publication.impactfactor | 2.794 | en_US |
Appears in Collections: | FS-NBFGR-Publication |
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