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http://krishi.icar.gov.in/jspui/handle/123456789/50988| Title: | Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications |
| Other Titles: | Not Available |
| Authors: | Neha Singh Pankaj Soni Basdeo Kushwaha Murali Sanjeev Kumar J. K. Srivastava Shreya Srivastava Akhilesh Kumar Mishra Ravindra Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Bureau of Fish Genetic Resources, Lucknow AMITY University, Uttar Pradesh, Lucknow Campus, Lucknow |
| Published/ Complete Date: | 2021-01-01 |
| Project Code: | Not Available |
| Keywords: | Cell line Clarias magur Cytotoxicity Heavy metals Testis |
| Publisher: | Springer India |
| Citation: | Singh, N., Soni, P., Kushwaha, B., Kumar, M.S., Srivastava, J.K., Srivastava, S., Mishra, A.K. and Kumar, R., 2021. Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications. The Nucleus,64:211–217 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | We developed a gonad cell line, CMgT-1, from the testes of Clarias magur in L-15 media with 20% FBS at 28 °C temperature and sub-cultured it more than 50 times. The origin of the cell line was validated using two universal markers, viz. cytochrome oxidase c subunit I and 16S rRNA genes. Cytogenetic analysis of the cells at 32nd passage revealed diploid chromosome number of 50. Immuno-phenotyping assay confirmed an epithelial-like cell type. The cell line was tested for mycoplasma contamination and found to be negative. The cells were successfully transfected with a GFP reporter gene that indicated that the cell line could be utilized in gene expression studies. The cell line was successfully utilized for evaluating cytotoxic effects of heavy metals, viz. potassium dichromate, mercury chloride and sodium (meta) arsenite, which reflects its potential applications in toxicity studies. CMgT-1 cell line will serve as a valuable resource for cytotoxicity and other in vitro studies. |
| Description: | Not Available |
| ISSN: | 0029-568X (Print ) 0976-7975 (Electronic) |
| Type(s) of content: | Research Paper |
| Sponsors: | Department of Biotechnology, Government of India, New Delhi, India, vide Sanction Order No. BT/PR13908/ AAQ/3/733/2015 |
| Language: | English |
| Name of Journal: | The Nucleus |
| Journal Type: | This Journal ‘The Nucleus’ publishes original research and review articles on novel findings on chromosomes and cell genetics, including genetic diversity, mutagenesis, genotoxicity, genomics, proteomics, metabolomics and bioinformatics with implications on evolution, species diversification, epigenetical regulations and all related facets dealing with enrichment of knowledge and human welfare. |
| Impact Factor: | - |
| Volume No.: | 64 |
| Page Number: | 211-217 |
| Name of the Division/Regional Station: | Molecular Biology and Biotechnology |
| Source, DOI or any other URL: | 10.1007/s13237-020-00345-w https://link.springer.com/article/10.1007/s13237-020-00345-w |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50988 |
| Appears in Collections: | FS-NBFGR-Publication |
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