KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/5114| Title: | Isolation and characterization of outer membrane proteins of Edwardsiella tarda and its application in immunoassays |
| Other Titles: | Not Available |
| Authors: | Gokhlesh Kumar Gaurav Rathore U. Sengupta V. Singh D. Kapoor W.S. Lakra |
| Published/ Complete Date: | 2007-11 |
| Project Code: | Not Available |
| Keywords: | Edwardsiella tarda Outer membrane proteins Western blotting ELISA |
| Publisher: | Elsevier |
| Citation: | Kumar, G., Rathore, G., Sengupta, U., Singh, V., Kapoor, D. and Lakra, W.S., 2007. Isolation and characterization of outer membrane proteins of Edwardsiella tarda and its application in immunoassays. Aquaculture, 272(1), pp.98-104. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and also for gastro intestinal infections in humans.Sodium lauryl sarcosinate extraction method was used for the isolation of outer membrane proteins (OMPs) from 16 isolates of E. tarda, recovered from snakeheads (Ophiocephalus punctatus). Sodium dodecyl sulphate polyacrylamide gel electrophoresis profiles of OMPs from E. tarda isolates had one major and common protein band of 44 kilodalton (kDa) and 9 other variable protein bands. Rabbit polyclonal antibodies (RPAbs) against OMPs of E. tarda ET-7 detected mainly one common 44 kDa protein in all isolates by western blotting. RPAbs strongly reacted with all isolates of E. tarda by indirect enzyme linked immunosorbent assay (ELISA). These polyclonal antibodies showed no cross reactivity with OMPs or whole cells of other selected aquatic bacteria, i.e., Escherichia coli, Pseudomonas fluorescens, Aeromonas hydrophila, Vibrio cholerae and Flavobacterium ferrugineum. This study indicated that RPAbs against OMPs is useful for detection of E. tarda by ELISA . |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Aquaculture |
| Volume No.: | 272 |
| Page Number: | 98–104 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1016/j.aquaculture.2007.08.054 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5114 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Isolation-E. tarda.pdf | 847.6 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.